Recruitment of a foreign quinone into the A1 site of photosystem I: In vivo replacement of plastoquinone-9 by media-supplemented naphthoquinones in phylloquinone biosynthetic pathway mutants of synechocystis sp. PCC 6803

T. Wade Johnson, Boris Zybailov, A. Daniel Jones, Robert Bittl, Stephan Zech, Dietmar Stehlik, John H. Golbeck, Parag R. Chitnis

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Abstract

Interruption of the phylloquinone (PhQ) biosynthetic pathway by interposon mutagenesis of the mena and menB genes in Synechocystis sp. PCC 6803 results in plastoquinone-9 (PQ-9) occupying the A1 site and functioning in electron transfer from A1 to the FeS clusters in photosystem (PS) I (Johnson, T. W., Shen, G., Zybailov, B., Kolling, D., Reategui, R., Beauparlant, S., Vassiliev, I. R., Bryant, D. A., Jones, A. D., Golbeck, J. H., and Chitnis, P. R. (2000) J. Biol. Chem. 275, 8523-8530. We report here the isolation of menB26, a strain of the menB mutant that grows in high light by virtue of a higher PS I to PS II ratio. PhQ can be reincorporated into the A1 site of the menB26 mutant strain by supplementing the growth medium with authentic PhQ. The reincorporation of PhQ also occurs in cells that have been treated with protein synthesis inhibitors, consistent with a displacement of PQ-9 from the A1 site by mass action. The doubling time of the menB26 mutant cells, but not the mena mutant cells, approaches the wild type when the growth medium is supplemented with naphthoquinone (NQ) derivatives such as 2-CO2H-1,4-NQ and 2-CH3-1,4-NQ. Since PhQ replaces PQ-9 in the supplemented menB26 mutant cells, but not in the mena mutant cells, the phytyl tail accompanies the incorporation of these quinones into the A1 site. Studies with menB26 mutant cells and perdeuterated 2-CH3-1,4-NQ shows that phytylation occurs at position 3 of the NQ ring because the deuterated 2-methyl group remains intact. Therefore, the specificity of the phytyltransferase enzyme is selective with respect to the group present at ring positions 2 and 3. Supplementing the growth medium of menB26 mutant cells with 1,4-NQ also leads to its incorporation into the A 1 site, but typically without either the phytyl tail or the methyl group. These findings open the possibility of biologically incorporating novel quinones into the A1 site by supplementing the growth medium of menB26 mutant cells.

Original languageEnglish (US)
Pages (from-to)39512-39521
Number of pages10
JournalJournal of Biological Chemistry
Volume276
Issue number43
DOIs
StatePublished - Oct 26 2001

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Plastoquinone
Vitamin K 1
Synechocystis
Naphthoquinones
Photosystem I Protein Complex
Biosynthetic Pathways
Quinones
Growth
Mutagenesis
Protein Synthesis Inhibitors
Photosystem II Protein Complex
Genes
benzoquinone
Derivatives
1,4-naphthoquinone
Electrons
Enzymes
Light

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

@article{134ea43d52004214baca905632d9e0bd,
title = "Recruitment of a foreign quinone into the A1 site of photosystem I: In vivo replacement of plastoquinone-9 by media-supplemented naphthoquinones in phylloquinone biosynthetic pathway mutants of synechocystis sp. PCC 6803",
abstract = "Interruption of the phylloquinone (PhQ) biosynthetic pathway by interposon mutagenesis of the mena and menB genes in Synechocystis sp. PCC 6803 results in plastoquinone-9 (PQ-9) occupying the A1 site and functioning in electron transfer from A1 to the FeS clusters in photosystem (PS) I (Johnson, T. W., Shen, G., Zybailov, B., Kolling, D., Reategui, R., Beauparlant, S., Vassiliev, I. R., Bryant, D. A., Jones, A. D., Golbeck, J. H., and Chitnis, P. R. (2000) J. Biol. Chem. 275, 8523-8530. We report here the isolation of menB26, a strain of the menB mutant that grows in high light by virtue of a higher PS I to PS II ratio. PhQ can be reincorporated into the A1 site of the menB26 mutant strain by supplementing the growth medium with authentic PhQ. The reincorporation of PhQ also occurs in cells that have been treated with protein synthesis inhibitors, consistent with a displacement of PQ-9 from the A1 site by mass action. The doubling time of the menB26 mutant cells, but not the mena mutant cells, approaches the wild type when the growth medium is supplemented with naphthoquinone (NQ) derivatives such as 2-CO2H-1,4-NQ and 2-CH3-1,4-NQ. Since PhQ replaces PQ-9 in the supplemented menB26 mutant cells, but not in the mena mutant cells, the phytyl tail accompanies the incorporation of these quinones into the A1 site. Studies with menB26 mutant cells and perdeuterated 2-CH3-1,4-NQ shows that phytylation occurs at position 3 of the NQ ring because the deuterated 2-methyl group remains intact. Therefore, the specificity of the phytyltransferase enzyme is selective with respect to the group present at ring positions 2 and 3. Supplementing the growth medium of menB26 mutant cells with 1,4-NQ also leads to its incorporation into the A 1 site, but typically without either the phytyl tail or the methyl group. These findings open the possibility of biologically incorporating novel quinones into the A1 site by supplementing the growth medium of menB26 mutant cells.",
author = "Johnson, {T. Wade} and Boris Zybailov and Jones, {A. Daniel} and Robert Bittl and Stephan Zech and Dietmar Stehlik and Golbeck, {John H.} and Chitnis, {Parag R.}",
year = "2001",
month = "10",
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doi = "10.1074/jbc.M104040200",
language = "English (US)",
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journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
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}

Recruitment of a foreign quinone into the A1 site of photosystem I : In vivo replacement of plastoquinone-9 by media-supplemented naphthoquinones in phylloquinone biosynthetic pathway mutants of synechocystis sp. PCC 6803. / Johnson, T. Wade; Zybailov, Boris; Jones, A. Daniel; Bittl, Robert; Zech, Stephan; Stehlik, Dietmar; Golbeck, John H.; Chitnis, Parag R.

In: Journal of Biological Chemistry, Vol. 276, No. 43, 26.10.2001, p. 39512-39521.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Recruitment of a foreign quinone into the A1 site of photosystem I

T2 - In vivo replacement of plastoquinone-9 by media-supplemented naphthoquinones in phylloquinone biosynthetic pathway mutants of synechocystis sp. PCC 6803

AU - Johnson, T. Wade

AU - Zybailov, Boris

AU - Jones, A. Daniel

AU - Bittl, Robert

AU - Zech, Stephan

AU - Stehlik, Dietmar

AU - Golbeck, John H.

AU - Chitnis, Parag R.

PY - 2001/10/26

Y1 - 2001/10/26

N2 - Interruption of the phylloquinone (PhQ) biosynthetic pathway by interposon mutagenesis of the mena and menB genes in Synechocystis sp. PCC 6803 results in plastoquinone-9 (PQ-9) occupying the A1 site and functioning in electron transfer from A1 to the FeS clusters in photosystem (PS) I (Johnson, T. W., Shen, G., Zybailov, B., Kolling, D., Reategui, R., Beauparlant, S., Vassiliev, I. R., Bryant, D. A., Jones, A. D., Golbeck, J. H., and Chitnis, P. R. (2000) J. Biol. Chem. 275, 8523-8530. We report here the isolation of menB26, a strain of the menB mutant that grows in high light by virtue of a higher PS I to PS II ratio. PhQ can be reincorporated into the A1 site of the menB26 mutant strain by supplementing the growth medium with authentic PhQ. The reincorporation of PhQ also occurs in cells that have been treated with protein synthesis inhibitors, consistent with a displacement of PQ-9 from the A1 site by mass action. The doubling time of the menB26 mutant cells, but not the mena mutant cells, approaches the wild type when the growth medium is supplemented with naphthoquinone (NQ) derivatives such as 2-CO2H-1,4-NQ and 2-CH3-1,4-NQ. Since PhQ replaces PQ-9 in the supplemented menB26 mutant cells, but not in the mena mutant cells, the phytyl tail accompanies the incorporation of these quinones into the A1 site. Studies with menB26 mutant cells and perdeuterated 2-CH3-1,4-NQ shows that phytylation occurs at position 3 of the NQ ring because the deuterated 2-methyl group remains intact. Therefore, the specificity of the phytyltransferase enzyme is selective with respect to the group present at ring positions 2 and 3. Supplementing the growth medium of menB26 mutant cells with 1,4-NQ also leads to its incorporation into the A 1 site, but typically without either the phytyl tail or the methyl group. These findings open the possibility of biologically incorporating novel quinones into the A1 site by supplementing the growth medium of menB26 mutant cells.

AB - Interruption of the phylloquinone (PhQ) biosynthetic pathway by interposon mutagenesis of the mena and menB genes in Synechocystis sp. PCC 6803 results in plastoquinone-9 (PQ-9) occupying the A1 site and functioning in electron transfer from A1 to the FeS clusters in photosystem (PS) I (Johnson, T. W., Shen, G., Zybailov, B., Kolling, D., Reategui, R., Beauparlant, S., Vassiliev, I. R., Bryant, D. A., Jones, A. D., Golbeck, J. H., and Chitnis, P. R. (2000) J. Biol. Chem. 275, 8523-8530. We report here the isolation of menB26, a strain of the menB mutant that grows in high light by virtue of a higher PS I to PS II ratio. PhQ can be reincorporated into the A1 site of the menB26 mutant strain by supplementing the growth medium with authentic PhQ. The reincorporation of PhQ also occurs in cells that have been treated with protein synthesis inhibitors, consistent with a displacement of PQ-9 from the A1 site by mass action. The doubling time of the menB26 mutant cells, but not the mena mutant cells, approaches the wild type when the growth medium is supplemented with naphthoquinone (NQ) derivatives such as 2-CO2H-1,4-NQ and 2-CH3-1,4-NQ. Since PhQ replaces PQ-9 in the supplemented menB26 mutant cells, but not in the mena mutant cells, the phytyl tail accompanies the incorporation of these quinones into the A1 site. Studies with menB26 mutant cells and perdeuterated 2-CH3-1,4-NQ shows that phytylation occurs at position 3 of the NQ ring because the deuterated 2-methyl group remains intact. Therefore, the specificity of the phytyltransferase enzyme is selective with respect to the group present at ring positions 2 and 3. Supplementing the growth medium of menB26 mutant cells with 1,4-NQ also leads to its incorporation into the A 1 site, but typically without either the phytyl tail or the methyl group. These findings open the possibility of biologically incorporating novel quinones into the A1 site by supplementing the growth medium of menB26 mutant cells.

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