Recruitment of coregulator complexes to the β-globin gene locus by TFII-I and upstream stimulatory factor

Valerie J. Crusselle-Davis, Zhuo Zhou, Archana Anantharaman, Babak Moghimi, Tihomir Dodev, Suming Huang, Jörg Bungert

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Upstream stimulatory factor and TFII-I are ubiquitously expressed helix-loop-helix transcription factors that interact with E-box sequences and or initiator elements. We previously demonstrated that upstream stimulatory factor is an activator of β-globin gene expression whereas TFII-I is a repressor. In the present study, we demonstrate that upstream stimulatory factor interacts with the coactivator p300 and that this interaction is restricted to erythroid cells expressing the adult β-globin gene. Furthermore, we demonstrate that Suz12, a component of the polycomb repressor complex 2, is recruited to the β-globin gene. Reducing expression of Suz12 significantly activates β-globin gene expression in an erythroid cell line with an embryonic phenotype. Suz12 also interacts with the adult β-globin gene during early stages of erythroid differentiation of mouse embryonic stem cells. Our data suggest that TFII-I contributes to the recruitment of the polycomb repressor complex 2 complex to the β-globin gene. Together, these data demonstrate that the antagonistic activities of upstream stimulatory factor and TFII-I on β-globin gene expression are mediated at least in part by protein complexes that render the promoter associated chromatin accessible or inaccessible for the transcription complex.

Original languageEnglish (US)
Pages (from-to)6065-6073
Number of pages9
JournalFEBS Journal
Volume274
Issue number23
DOIs
StatePublished - Dec 1 2007

Fingerprint

Upstream Stimulatory Factors
Globins
Genes
Gene expression
Erythroid Cells
Gene Expression
Cells
E-Box Elements
Transcription
Stem cells
Chromatin
Transcription Factors
Phenotype
Cell Line

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Crusselle-Davis, V. J., Zhou, Z., Anantharaman, A., Moghimi, B., Dodev, T., Huang, S., & Bungert, J. (2007). Recruitment of coregulator complexes to the β-globin gene locus by TFII-I and upstream stimulatory factor. FEBS Journal, 274(23), 6065-6073. https://doi.org/10.1111/j.1742-4658.2007.06128.x
Crusselle-Davis, Valerie J. ; Zhou, Zhuo ; Anantharaman, Archana ; Moghimi, Babak ; Dodev, Tihomir ; Huang, Suming ; Bungert, Jörg. / Recruitment of coregulator complexes to the β-globin gene locus by TFII-I and upstream stimulatory factor. In: FEBS Journal. 2007 ; Vol. 274, No. 23. pp. 6065-6073.
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Crusselle-Davis, VJ, Zhou, Z, Anantharaman, A, Moghimi, B, Dodev, T, Huang, S & Bungert, J 2007, 'Recruitment of coregulator complexes to the β-globin gene locus by TFII-I and upstream stimulatory factor', FEBS Journal, vol. 274, no. 23, pp. 6065-6073. https://doi.org/10.1111/j.1742-4658.2007.06128.x

Recruitment of coregulator complexes to the β-globin gene locus by TFII-I and upstream stimulatory factor. / Crusselle-Davis, Valerie J.; Zhou, Zhuo; Anantharaman, Archana; Moghimi, Babak; Dodev, Tihomir; Huang, Suming; Bungert, Jörg.

In: FEBS Journal, Vol. 274, No. 23, 01.12.2007, p. 6065-6073.

Research output: Contribution to journalArticle

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