Recruitment of HAT complexes by direct activator interactions with the ATM-related Tra1 subunit

C. E. Brown, L. Howe, K. Sousa, S. C. Alley, M. J. Carrozza, Song Tan, J. L. Workman

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Abstract

Promoter-specific recruitment of histone acetyltransferase activity is often critical for transcriptional activation. We present a detailed study of the interaction between the histone acetyltransferase complexes SAGA and NuA4, and transcription activators. We demonstrate by affinity chromatography and photo-cross-linking label transfer that acidic activators directly interact with Tra1p, a shared subunit of SAGA and NuA4. Mutations within the COOH-terminus of Tra1p disrupted its interaction with activators and resulted in gene-specific transcriptional defects that correlated with lowered promoter-specific histone acetylation. These data demonstrate that the essential Tra1 protein serves as a common target for activators in both SAGA and NuA4 acetyltransferases.

Original languageEnglish (US)
Pages (from-to)2333-2337
Number of pages5
JournalScience
Volume292
Issue number5525
DOIs
Publication statusPublished - Jun 22 2001

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All Science Journal Classification (ASJC) codes

  • General

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Brown, C. E., Howe, L., Sousa, K., Alley, S. C., Carrozza, M. J., Tan, S., & Workman, J. L. (2001). Recruitment of HAT complexes by direct activator interactions with the ATM-related Tra1 subunit. Science, 292(5525), 2333-2337. https://doi.org/10.1126/science.1060214