Redistribution of labile plasma zinc during mild surgical stress in the rat

Edward Kelly, Jeffrey Mathew, Jonathan E. Kohler, Amy L. Blass, David I. Soybel

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Zinc is an essential trace element and cofactor for many cellular processes. Uptake of ionized divalent zinc (Zn2+) in peripheral tissues depends on its total content in the circulation and on mechanisms facilitating delivery to tissues in its labile form. Understanding mechanisms of Zn2+ delivery has been hindered by the absence of techniques to detect labile Zn2+ in the circulation. In this study, we report the use of the fluorescent zinc-binding dye (ZnAF-2) to detect changes in labile Zn2+ in the circulating plasma of the rat under standardized conditions, including exogenous infusions to increase plasma Zn2+ and an infusion of the chelator, citrate, to decrease labile Zn2+ in the plasma without altering total Zn2+ content. In a model of mild surgical stress (unilateral femoral arterial ligation), plasma levels of total and labile Zn2+ decreased significantly 24 h after the operation. Ultrafiltration of plasma into high- and low-molecular weight macromolecule fractionations indicated that binding capacity of zinc in the high-molecular weight fraction is impaired for the entire 24-h interval after induction of mild surgical stress. Affinity of the filtrate fraction was rapidly and reversibly responsive to anesthesia alone, decreasing significantly at 4 h and recovering at 24 h; in animals subjected to moderate surgical stress, this responsiveness was lost. These findings are the first reported measurements of labile Zn 2+ in the circulation in any form of mild systemic stress. Zinc undergoes substantial redistribution in the plasma as a response to surgical stress, leading to increased availability in lower molecular weight fractions and in its labile form.

Original languageEnglish (US)
Pages (from-to)139-149
Number of pages11
JournalTranslational Research
Volume157
Issue number3
DOIs
StatePublished - Mar 2011

All Science Journal Classification (ASJC) codes

  • Public Health, Environmental and Occupational Health
  • Biochemistry, medical
  • Physiology (medical)

Fingerprint Dive into the research topics of 'Redistribution of labile plasma zinc during mild surgical stress in the rat'. Together they form a unique fingerprint.

Cite this