Reduced competitive repopulation capacity of multipotential hematopoietic stem cells in the bone marrow of friend virus-infected Fv2-resistant mice

Michael W. Epperly, Xichen Zhang, Renee Fisher, Wen Hou, Darcy Franicola, Donna Shields, Michael Quickel, Pamela Hankey Giblin, Hong Wang, Joel S. Greenberger

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background/Aim: The polycythemia form of Friend leukemia virus (FVP) causes splenomegaly and lethal erythroleukemia in Fv-2s-susceptible mouse strains. We sought to determine whether the hematopoietic stem cell (HSC) pool was expanded in Fv-2r-resistant mice. Materials and Methods: The 120-day bone marrow transplantation competitive repopulation assay was used to determine whether FVP-infected Fv-2r C57BL/6 mice demonstrated expansion of the HSC pool compared to the pool of committed hematopoietic progenitor cells in the same marrow assayed in vitro. Results: There was a significant expansion of committed hematopoietic progenitors observed in virus-infected Fv-2s FVB mice, but not Fv-2r C57BL/6 mice. Furthermore, Fv-2r mice showed no detectable expansion of either committed hematopoietic progenitor cells or the multipotential stem cell pool by competitive repopulation assay. Conclusion: Friend virus disease in Fv-2s mice is associated with expansion of committed hematopoietic progenitors. Fv-2r mice show no expansion of either committed progenitor or pluripotential stem cell numbers.

Original languageEnglish (US)
Pages (from-to)313-320
Number of pages8
JournalIn Vivo
Volume31
Issue number3
DOIs
StatePublished - May 1 2017

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Friend murine leukemia virus
Hematopoietic Stem Cells
Stem cells
Viruses
Bone
Bone Marrow
Assays
Inbred C57BL Mouse
Stem Cells
Leukemia, Erythroblastic, Acute
Polycythemia
Splenomegaly
Virus Diseases
Bone Marrow Transplantation
Leukemia
Cell Count

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

Cite this

Epperly, Michael W. ; Zhang, Xichen ; Fisher, Renee ; Hou, Wen ; Franicola, Darcy ; Shields, Donna ; Quickel, Michael ; Giblin, Pamela Hankey ; Wang, Hong ; Greenberger, Joel S. / Reduced competitive repopulation capacity of multipotential hematopoietic stem cells in the bone marrow of friend virus-infected Fv2-resistant mice. In: In Vivo. 2017 ; Vol. 31, No. 3. pp. 313-320.
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abstract = "Background/Aim: The polycythemia form of Friend leukemia virus (FVP) causes splenomegaly and lethal erythroleukemia in Fv-2s-susceptible mouse strains. We sought to determine whether the hematopoietic stem cell (HSC) pool was expanded in Fv-2r-resistant mice. Materials and Methods: The 120-day bone marrow transplantation competitive repopulation assay was used to determine whether FVP-infected Fv-2r C57BL/6 mice demonstrated expansion of the HSC pool compared to the pool of committed hematopoietic progenitor cells in the same marrow assayed in vitro. Results: There was a significant expansion of committed hematopoietic progenitors observed in virus-infected Fv-2s FVB mice, but not Fv-2r C57BL/6 mice. Furthermore, Fv-2r mice showed no detectable expansion of either committed hematopoietic progenitor cells or the multipotential stem cell pool by competitive repopulation assay. Conclusion: Friend virus disease in Fv-2s mice is associated with expansion of committed hematopoietic progenitors. Fv-2r mice show no expansion of either committed progenitor or pluripotential stem cell numbers.",
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Epperly, MW, Zhang, X, Fisher, R, Hou, W, Franicola, D, Shields, D, Quickel, M, Giblin, PH, Wang, H & Greenberger, JS 2017, 'Reduced competitive repopulation capacity of multipotential hematopoietic stem cells in the bone marrow of friend virus-infected Fv2-resistant mice', In Vivo, vol. 31, no. 3, pp. 313-320. https://doi.org/10.21873/invivo.11061

Reduced competitive repopulation capacity of multipotential hematopoietic stem cells in the bone marrow of friend virus-infected Fv2-resistant mice. / Epperly, Michael W.; Zhang, Xichen; Fisher, Renee; Hou, Wen; Franicola, Darcy; Shields, Donna; Quickel, Michael; Giblin, Pamela Hankey; Wang, Hong; Greenberger, Joel S.

In: In Vivo, Vol. 31, No. 3, 01.05.2017, p. 313-320.

Research output: Contribution to journalArticle

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AU - Epperly, Michael W.

AU - Zhang, Xichen

AU - Fisher, Renee

AU - Hou, Wen

AU - Franicola, Darcy

AU - Shields, Donna

AU - Quickel, Michael

AU - Giblin, Pamela Hankey

AU - Wang, Hong

AU - Greenberger, Joel S.

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N2 - Background/Aim: The polycythemia form of Friend leukemia virus (FVP) causes splenomegaly and lethal erythroleukemia in Fv-2s-susceptible mouse strains. We sought to determine whether the hematopoietic stem cell (HSC) pool was expanded in Fv-2r-resistant mice. Materials and Methods: The 120-day bone marrow transplantation competitive repopulation assay was used to determine whether FVP-infected Fv-2r C57BL/6 mice demonstrated expansion of the HSC pool compared to the pool of committed hematopoietic progenitor cells in the same marrow assayed in vitro. Results: There was a significant expansion of committed hematopoietic progenitors observed in virus-infected Fv-2s FVB mice, but not Fv-2r C57BL/6 mice. Furthermore, Fv-2r mice showed no detectable expansion of either committed hematopoietic progenitor cells or the multipotential stem cell pool by competitive repopulation assay. Conclusion: Friend virus disease in Fv-2s mice is associated with expansion of committed hematopoietic progenitors. Fv-2r mice show no expansion of either committed progenitor or pluripotential stem cell numbers.

AB - Background/Aim: The polycythemia form of Friend leukemia virus (FVP) causes splenomegaly and lethal erythroleukemia in Fv-2s-susceptible mouse strains. We sought to determine whether the hematopoietic stem cell (HSC) pool was expanded in Fv-2r-resistant mice. Materials and Methods: The 120-day bone marrow transplantation competitive repopulation assay was used to determine whether FVP-infected Fv-2r C57BL/6 mice demonstrated expansion of the HSC pool compared to the pool of committed hematopoietic progenitor cells in the same marrow assayed in vitro. Results: There was a significant expansion of committed hematopoietic progenitors observed in virus-infected Fv-2s FVB mice, but not Fv-2r C57BL/6 mice. Furthermore, Fv-2r mice showed no detectable expansion of either committed hematopoietic progenitor cells or the multipotential stem cell pool by competitive repopulation assay. Conclusion: Friend virus disease in Fv-2s mice is associated with expansion of committed hematopoietic progenitors. Fv-2r mice show no expansion of either committed progenitor or pluripotential stem cell numbers.

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