Reduced competitive repopulation capacity of multipotential hematopoietic stem cells in the bone marrow of friend virus-infected Fv2-resistant mice

Michael W. Epperly, Xichen Zhang, Renee Fisher, Wen Hou, Darcy Franicola, Donna Shields, Michael Quickel, Pamela Hankey-Giblin, Hong Wang, Joel S. Greenberger

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Background/Aim: The polycythemia form of Friend leukemia virus (FVP) causes splenomegaly and lethal erythroleukemia in Fv-2s-susceptible mouse strains. We sought to determine whether the hematopoietic stem cell (HSC) pool was expanded in Fv-2r-resistant mice. Materials and Methods: The 120-day bone marrow transplantation competitive repopulation assay was used to determine whether FVP-infected Fv-2r C57BL/6 mice demonstrated expansion of the HSC pool compared to the pool of committed hematopoietic progenitor cells in the same marrow assayed in vitro. Results: There was a significant expansion of committed hematopoietic progenitors observed in virus-infected Fv-2s FVB mice, but not Fv-2r C57BL/6 mice. Furthermore, Fv-2r mice showed no detectable expansion of either committed hematopoietic progenitor cells or the multipotential stem cell pool by competitive repopulation assay. Conclusion: Friend virus disease in Fv-2s mice is associated with expansion of committed hematopoietic progenitors. Fv-2r mice show no expansion of either committed progenitor or pluripotential stem cell numbers.

Original languageEnglish (US)
Pages (from-to)313-320
Number of pages8
JournalIn Vivo
Volume31
Issue number3
DOIs
StatePublished - May 1 2017

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

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    Epperly, M. W., Zhang, X., Fisher, R., Hou, W., Franicola, D., Shields, D., Quickel, M., Hankey-Giblin, P., Wang, H., & Greenberger, J. S. (2017). Reduced competitive repopulation capacity of multipotential hematopoietic stem cells in the bone marrow of friend virus-infected Fv2-resistant mice. In Vivo, 31(3), 313-320. https://doi.org/10.21873/invivo.11061