Reduced oxygen tension induces pulmonary endothelium to release a pulmonary smooth muscle cell mitogen(s)

Robert Vender, D. R. Clemmons, L. Kwock, M. Friedman

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Bovine pulmonary endothelial cells grown in vitro were shown to release a factor that was mitogenic for pulmonary smooth muscle cells during exposure to a reduced oxygen tension atmosphere. The addition of hypoxic endothelium-derived medium resulted in a 60% increase in smooth muscle cell number after 24 h of exposure. Addition of medium from pulmonary endothelium exposed to normoxic conditions or medium derived from either hypoxic or normoxic aortic endothelium did not result in significant increases in smooth muscle cell number. Physicochemical characterization of the hypoxic pulmonary endothelial cell-derived factor(s) showed that it was resistant to heat and reducing agent treatment and stable between pH 3 and 10. The mitogenic activity decreased by 71% at pH 2 and by 68% after treatment with trypsin. The activity adhered to DEAE Sephadex. Gel filtration chromatography of the hypoxic-conditioned medium demonstrated 2 major peaks of smooth muscle cell growth factor activity corresponding to molecular weights between 6,000 and 14,000 and 20,000 and 65,000 daltons, respectively. These data suggest that this pulmonary endothelial cell-derived smooth muscle cell peptide mitogen(s) may be involved in the smooth muscle cell proliferative response seen with chronic alveolar hypoxia.

Original languageEnglish (US)
Pages (from-to)622-627
Number of pages6
JournalAmerican Review of Respiratory Disease
Volume135
Issue number3
StatePublished - Jan 1 1987

Fingerprint

Mitogens
Smooth Muscle Myocytes
Endothelium
Oxygen
Lung
Endothelial Cells
Cell Count
DEAE-Dextran
Reducing Agents
Conditioned Culture Medium
Atmosphere
Trypsin
Gel Chromatography
Intercellular Signaling Peptides and Proteins
Hot Temperature
Molecular Weight
Peptides

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine

Cite this

@article{606d89e883a147fd91143047883533e4,
title = "Reduced oxygen tension induces pulmonary endothelium to release a pulmonary smooth muscle cell mitogen(s)",
abstract = "Bovine pulmonary endothelial cells grown in vitro were shown to release a factor that was mitogenic for pulmonary smooth muscle cells during exposure to a reduced oxygen tension atmosphere. The addition of hypoxic endothelium-derived medium resulted in a 60{\%} increase in smooth muscle cell number after 24 h of exposure. Addition of medium from pulmonary endothelium exposed to normoxic conditions or medium derived from either hypoxic or normoxic aortic endothelium did not result in significant increases in smooth muscle cell number. Physicochemical characterization of the hypoxic pulmonary endothelial cell-derived factor(s) showed that it was resistant to heat and reducing agent treatment and stable between pH 3 and 10. The mitogenic activity decreased by 71{\%} at pH 2 and by 68{\%} after treatment with trypsin. The activity adhered to DEAE Sephadex. Gel filtration chromatography of the hypoxic-conditioned medium demonstrated 2 major peaks of smooth muscle cell growth factor activity corresponding to molecular weights between 6,000 and 14,000 and 20,000 and 65,000 daltons, respectively. These data suggest that this pulmonary endothelial cell-derived smooth muscle cell peptide mitogen(s) may be involved in the smooth muscle cell proliferative response seen with chronic alveolar hypoxia.",
author = "Robert Vender and Clemmons, {D. R.} and L. Kwock and M. Friedman",
year = "1987",
month = "1",
day = "1",
language = "English (US)",
volume = "135",
pages = "622--627",
journal = "American Journal of Respiratory and Critical Care Medicine",
issn = "1073-449X",
publisher = "American Thoracic Society",
number = "3",

}

Reduced oxygen tension induces pulmonary endothelium to release a pulmonary smooth muscle cell mitogen(s). / Vender, Robert; Clemmons, D. R.; Kwock, L.; Friedman, M.

In: American Review of Respiratory Disease, Vol. 135, No. 3, 01.01.1987, p. 622-627.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Reduced oxygen tension induces pulmonary endothelium to release a pulmonary smooth muscle cell mitogen(s)

AU - Vender, Robert

AU - Clemmons, D. R.

AU - Kwock, L.

AU - Friedman, M.

PY - 1987/1/1

Y1 - 1987/1/1

N2 - Bovine pulmonary endothelial cells grown in vitro were shown to release a factor that was mitogenic for pulmonary smooth muscle cells during exposure to a reduced oxygen tension atmosphere. The addition of hypoxic endothelium-derived medium resulted in a 60% increase in smooth muscle cell number after 24 h of exposure. Addition of medium from pulmonary endothelium exposed to normoxic conditions or medium derived from either hypoxic or normoxic aortic endothelium did not result in significant increases in smooth muscle cell number. Physicochemical characterization of the hypoxic pulmonary endothelial cell-derived factor(s) showed that it was resistant to heat and reducing agent treatment and stable between pH 3 and 10. The mitogenic activity decreased by 71% at pH 2 and by 68% after treatment with trypsin. The activity adhered to DEAE Sephadex. Gel filtration chromatography of the hypoxic-conditioned medium demonstrated 2 major peaks of smooth muscle cell growth factor activity corresponding to molecular weights between 6,000 and 14,000 and 20,000 and 65,000 daltons, respectively. These data suggest that this pulmonary endothelial cell-derived smooth muscle cell peptide mitogen(s) may be involved in the smooth muscle cell proliferative response seen with chronic alveolar hypoxia.

AB - Bovine pulmonary endothelial cells grown in vitro were shown to release a factor that was mitogenic for pulmonary smooth muscle cells during exposure to a reduced oxygen tension atmosphere. The addition of hypoxic endothelium-derived medium resulted in a 60% increase in smooth muscle cell number after 24 h of exposure. Addition of medium from pulmonary endothelium exposed to normoxic conditions or medium derived from either hypoxic or normoxic aortic endothelium did not result in significant increases in smooth muscle cell number. Physicochemical characterization of the hypoxic pulmonary endothelial cell-derived factor(s) showed that it was resistant to heat and reducing agent treatment and stable between pH 3 and 10. The mitogenic activity decreased by 71% at pH 2 and by 68% after treatment with trypsin. The activity adhered to DEAE Sephadex. Gel filtration chromatography of the hypoxic-conditioned medium demonstrated 2 major peaks of smooth muscle cell growth factor activity corresponding to molecular weights between 6,000 and 14,000 and 20,000 and 65,000 daltons, respectively. These data suggest that this pulmonary endothelial cell-derived smooth muscle cell peptide mitogen(s) may be involved in the smooth muscle cell proliferative response seen with chronic alveolar hypoxia.

UR - http://www.scopus.com/inward/record.url?scp=0023135830&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023135830&partnerID=8YFLogxK

M3 - Article

C2 - 3826890

AN - SCOPUS:0023135830

VL - 135

SP - 622

EP - 627

JO - American Journal of Respiratory and Critical Care Medicine

JF - American Journal of Respiratory and Critical Care Medicine

SN - 1073-449X

IS - 3

ER -