Reducing Ribosome Biosynthesis Promotes Translation during Low Mg2+ Stress

Mauricio H. Pontes, Jinki Yeom, Eduardo A. Groisman

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

The synthesis of ribosomes is regulated by both amino acid abundance and the availability of ATP, which regenerates guanosine triphosphate (GTP), powers ribosomes, and promotes transcription of rRNA genes. We now report that bacteria supersede both of these controls when experiencing low cytosolic magnesium (Mg2+), a divalent cation essential for ribosome stabilization and for neutralization of ATP's negative charge. We uncover a regulatory circuit that responds to low cytosolic Mg2+ by promoting expression of proteins that import Mg2+ and lower ATP amounts. This response reduces the levels of ATP and ribosomes, making Mg2+ ions available for translation. Mutants defective in Mg2+ uptake and unable to reduce ATP levels accumulate non-functional ribosomal components and undergo translational arrest. Our findings establish a paradigm whereby cells reduce the amounts of translating ribosomes to carry out protein synthesis.

Original languageEnglish (US)
Pages (from-to)480-492
Number of pages13
JournalMolecular cell
Volume64
Issue number3
DOIs
StatePublished - Nov 3 2016

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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