Reducing saturated fat intake is associated with increased levels of LDL receptors on mononuclear cells in healthy men and women

Vikkie A. Mustad, Terry D. Etherton, Allen D. Cooper, Andrea Marie Mastro, Thomas A. Pearson, Satya S. Jonnalagadda, Penny Margaret Kris-Etherton

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Studies with animal models suggest that saturated fatty acids raise low density lipoprotein (LDL)-cholesterol levels by reducing LDL receptor- mediated clearance. To examine this directly in humans, we studied the effects of lowering dietary saturated fat on LDL-receptor abundance in peripheral mononuclear cells which reflects hepatic LDL-receptor status. Healthy males and females (n = 25) participating in the DELTA (Dietary Effects on Lipoproteins and Thrombogenic Activity) Study consumed three experimental diets in a randomized cross-over design. Diets provided 34% fat, 15% saturated fatty acids (Average American Diet); 29% fat, 9% saturated fatty acids (Step-One Diet); and 25% fat, and 6% saturated fatty acids (Low SAT Diet). Peripheral mononuclear cells were isolated from blood samples collected after 6 and 8 wk. An ELISA was used to quantify LDL-receptor protein in total cell membranes. LDL-receptor abundance increased by 10.5% after the Low SAT Diet (P < 0.05). This was associated with an 11.8% decrease in serum LDL-cholesterol (P < 0.05). A linear inverse relationship was observed between the percentage change in LDL-cholesterol and the percentage change in LDL-receptor abundance (r = -0.59; P < 0.01). In addition, LDL- receptor abundance also was correlated inversely (P < 0.001) with serum levels of LDL-cholesterol (r = -0.747) and apoB (r = =0.593). In summary, reducing dietary saturated fat is associated with an increase in LDL-receptor abundance of magnitude similar to the decrease in serum LDL-cholesterol. Thus, an important mechanism by which reductions in dietary saturated fatty acids decrease LDL-cholesterol in humans is through an increase in LDL- receptor number.

Original languageEnglish (US)
Pages (from-to)459-468
Number of pages10
JournalJournal of Lipid Research
Volume38
Issue number3
StatePublished - Mar 1 1997

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LDL Receptors
Fats
Nutrition
LDL Cholesterol
Diet
Fatty Acids
Dietary Fats
Serum
Apolipoproteins B
Cell membranes
Cross-Over Studies
Lipoproteins
Animals
Blood
Animal Models
Enzyme-Linked Immunosorbent Assay
Cell Membrane
Liver

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

Mustad, Vikkie A. ; Etherton, Terry D. ; Cooper, Allen D. ; Mastro, Andrea Marie ; Pearson, Thomas A. ; Jonnalagadda, Satya S. ; Kris-Etherton, Penny Margaret. / Reducing saturated fat intake is associated with increased levels of LDL receptors on mononuclear cells in healthy men and women. In: Journal of Lipid Research. 1997 ; Vol. 38, No. 3. pp. 459-468.
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abstract = "Studies with animal models suggest that saturated fatty acids raise low density lipoprotein (LDL)-cholesterol levels by reducing LDL receptor- mediated clearance. To examine this directly in humans, we studied the effects of lowering dietary saturated fat on LDL-receptor abundance in peripheral mononuclear cells which reflects hepatic LDL-receptor status. Healthy males and females (n = 25) participating in the DELTA (Dietary Effects on Lipoproteins and Thrombogenic Activity) Study consumed three experimental diets in a randomized cross-over design. Diets provided 34{\%} fat, 15{\%} saturated fatty acids (Average American Diet); 29{\%} fat, 9{\%} saturated fatty acids (Step-One Diet); and 25{\%} fat, and 6{\%} saturated fatty acids (Low SAT Diet). Peripheral mononuclear cells were isolated from blood samples collected after 6 and 8 wk. An ELISA was used to quantify LDL-receptor protein in total cell membranes. LDL-receptor abundance increased by 10.5{\%} after the Low SAT Diet (P < 0.05). This was associated with an 11.8{\%} decrease in serum LDL-cholesterol (P < 0.05). A linear inverse relationship was observed between the percentage change in LDL-cholesterol and the percentage change in LDL-receptor abundance (r = -0.59; P < 0.01). In addition, LDL- receptor abundance also was correlated inversely (P < 0.001) with serum levels of LDL-cholesterol (r = -0.747) and apoB (r = =0.593). In summary, reducing dietary saturated fat is associated with an increase in LDL-receptor abundance of magnitude similar to the decrease in serum LDL-cholesterol. Thus, an important mechanism by which reductions in dietary saturated fatty acids decrease LDL-cholesterol in humans is through an increase in LDL- receptor number.",
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Reducing saturated fat intake is associated with increased levels of LDL receptors on mononuclear cells in healthy men and women. / Mustad, Vikkie A.; Etherton, Terry D.; Cooper, Allen D.; Mastro, Andrea Marie; Pearson, Thomas A.; Jonnalagadda, Satya S.; Kris-Etherton, Penny Margaret.

In: Journal of Lipid Research, Vol. 38, No. 3, 01.03.1997, p. 459-468.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Reducing saturated fat intake is associated with increased levels of LDL receptors on mononuclear cells in healthy men and women

AU - Mustad, Vikkie A.

AU - Etherton, Terry D.

AU - Cooper, Allen D.

AU - Mastro, Andrea Marie

AU - Pearson, Thomas A.

AU - Jonnalagadda, Satya S.

AU - Kris-Etherton, Penny Margaret

PY - 1997/3/1

Y1 - 1997/3/1

N2 - Studies with animal models suggest that saturated fatty acids raise low density lipoprotein (LDL)-cholesterol levels by reducing LDL receptor- mediated clearance. To examine this directly in humans, we studied the effects of lowering dietary saturated fat on LDL-receptor abundance in peripheral mononuclear cells which reflects hepatic LDL-receptor status. Healthy males and females (n = 25) participating in the DELTA (Dietary Effects on Lipoproteins and Thrombogenic Activity) Study consumed three experimental diets in a randomized cross-over design. Diets provided 34% fat, 15% saturated fatty acids (Average American Diet); 29% fat, 9% saturated fatty acids (Step-One Diet); and 25% fat, and 6% saturated fatty acids (Low SAT Diet). Peripheral mononuclear cells were isolated from blood samples collected after 6 and 8 wk. An ELISA was used to quantify LDL-receptor protein in total cell membranes. LDL-receptor abundance increased by 10.5% after the Low SAT Diet (P < 0.05). This was associated with an 11.8% decrease in serum LDL-cholesterol (P < 0.05). A linear inverse relationship was observed between the percentage change in LDL-cholesterol and the percentage change in LDL-receptor abundance (r = -0.59; P < 0.01). In addition, LDL- receptor abundance also was correlated inversely (P < 0.001) with serum levels of LDL-cholesterol (r = -0.747) and apoB (r = =0.593). In summary, reducing dietary saturated fat is associated with an increase in LDL-receptor abundance of magnitude similar to the decrease in serum LDL-cholesterol. Thus, an important mechanism by which reductions in dietary saturated fatty acids decrease LDL-cholesterol in humans is through an increase in LDL- receptor number.

AB - Studies with animal models suggest that saturated fatty acids raise low density lipoprotein (LDL)-cholesterol levels by reducing LDL receptor- mediated clearance. To examine this directly in humans, we studied the effects of lowering dietary saturated fat on LDL-receptor abundance in peripheral mononuclear cells which reflects hepatic LDL-receptor status. Healthy males and females (n = 25) participating in the DELTA (Dietary Effects on Lipoproteins and Thrombogenic Activity) Study consumed three experimental diets in a randomized cross-over design. Diets provided 34% fat, 15% saturated fatty acids (Average American Diet); 29% fat, 9% saturated fatty acids (Step-One Diet); and 25% fat, and 6% saturated fatty acids (Low SAT Diet). Peripheral mononuclear cells were isolated from blood samples collected after 6 and 8 wk. An ELISA was used to quantify LDL-receptor protein in total cell membranes. LDL-receptor abundance increased by 10.5% after the Low SAT Diet (P < 0.05). This was associated with an 11.8% decrease in serum LDL-cholesterol (P < 0.05). A linear inverse relationship was observed between the percentage change in LDL-cholesterol and the percentage change in LDL-receptor abundance (r = -0.59; P < 0.01). In addition, LDL- receptor abundance also was correlated inversely (P < 0.001) with serum levels of LDL-cholesterol (r = -0.747) and apoB (r = =0.593). In summary, reducing dietary saturated fat is associated with an increase in LDL-receptor abundance of magnitude similar to the decrease in serum LDL-cholesterol. Thus, an important mechanism by which reductions in dietary saturated fatty acids decrease LDL-cholesterol in humans is through an increase in LDL- receptor number.

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