Regenerable affinity chromatography support

V. K. Joshi, K. M. Shahani, A. Kilara, Fred W. Wagner

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

A derivative of Sepharose4B, p-(N-acetyl-l-tyrosineazo)benzamidoethyl Sepharose4B, was synthesized and used for the selective immobilization of proteins. The azo bond of this derivative can be reduced to liberate p-aminobenzamidoethyl Sepharose 4B, which can be diazotized and recoupled to N-acetyl-l-tyrosine and subsequently to a fresh preparation of protein. This regenerable affinity chromatography support was regenerated successively through five cycles without detectable loss of functional groups.

Original languageEnglish (US)
Pages (from-to)11-18
Number of pages8
JournalJournal of Chromatography A
Volume176
Issue number1
DOIs
StatePublished - Aug 1 1979

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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