Regeneration and plantlet devel'pment from somatic tissues of Aristolochia fimbriata

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7 Citations (Scopus)

Abstract

Aristolochia fimbriata is a small herbaceous perennial in the basal angiosperm family Aristolochiaceae. The family contains diverse floral forms ranging from radial to monosymmetric flowers with a wide variety of insect pollinators. Additionally, Aristolochia species contain secondary metabolites that are important natural toxins and traditional medicines, and are critical to the reproduction of swallowtail butterflies. These characteristics, in combination with the small genome size and short life cycle of A. fimbriata, have prompted further development of this species as a model system to study the evolution of basal angiosperms. As a prerequisite for developing a genetic transformation procedure for Aristolochia, we developed protocols for in vitro plant multiplication, shoot organogenesis, rooting, and acclimation of tissue culture-derived plants. Two varieties of Aristolochia were multiplied in vitro and rooted with 100% efficiency. Shoot regeneration was achieved within 1 month of culture initiation from whole leaf, internodal stem, and petiole explants. The highest regeneration success (97%) was recorded for stem explants. Regenerated and rooted shoots were acclimated to greenhouse conditions and developed flowers within 4 weeks of transplanting.

Original languageEnglish (US)
Pages (from-to)105-114
Number of pages10
JournalPlant Cell, Tissue and Organ Culture
Volume98
Issue number1
DOIs
StatePublished - Jul 1 2009

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Aristolochia
plantlets
shoots
Angiospermae
explants
natural toxicants
Aristolochiaceae
flowers
Papilionidae
stems
genetic transformation
pollinating insects
traditional medicine
transplanting (plants)
organogenesis
secondary metabolites
tissue culture
rooting
life cycle (organisms)
acclimation

All Science Journal Classification (ASJC) codes

  • Horticulture

Cite this

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abstract = "Aristolochia fimbriata is a small herbaceous perennial in the basal angiosperm family Aristolochiaceae. The family contains diverse floral forms ranging from radial to monosymmetric flowers with a wide variety of insect pollinators. Additionally, Aristolochia species contain secondary metabolites that are important natural toxins and traditional medicines, and are critical to the reproduction of swallowtail butterflies. These characteristics, in combination with the small genome size and short life cycle of A. fimbriata, have prompted further development of this species as a model system to study the evolution of basal angiosperms. As a prerequisite for developing a genetic transformation procedure for Aristolochia, we developed protocols for in vitro plant multiplication, shoot organogenesis, rooting, and acclimation of tissue culture-derived plants. Two varieties of Aristolochia were multiplied in vitro and rooted with 100{\%} efficiency. Shoot regeneration was achieved within 1 month of culture initiation from whole leaf, internodal stem, and petiole explants. The highest regeneration success (97{\%}) was recorded for stem explants. Regenerated and rooted shoots were acclimated to greenhouse conditions and developed flowers within 4 weeks of transplanting.",
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AU - Bliss, Barbara J.

AU - Sheaffer, Lena Landherr

AU - Depamphilis, Claude Walker

AU - Ma, Hong

AU - Hu, Yi

AU - Maximova, Siela

PY - 2009/7/1

Y1 - 2009/7/1

N2 - Aristolochia fimbriata is a small herbaceous perennial in the basal angiosperm family Aristolochiaceae. The family contains diverse floral forms ranging from radial to monosymmetric flowers with a wide variety of insect pollinators. Additionally, Aristolochia species contain secondary metabolites that are important natural toxins and traditional medicines, and are critical to the reproduction of swallowtail butterflies. These characteristics, in combination with the small genome size and short life cycle of A. fimbriata, have prompted further development of this species as a model system to study the evolution of basal angiosperms. As a prerequisite for developing a genetic transformation procedure for Aristolochia, we developed protocols for in vitro plant multiplication, shoot organogenesis, rooting, and acclimation of tissue culture-derived plants. Two varieties of Aristolochia were multiplied in vitro and rooted with 100% efficiency. Shoot regeneration was achieved within 1 month of culture initiation from whole leaf, internodal stem, and petiole explants. The highest regeneration success (97%) was recorded for stem explants. Regenerated and rooted shoots were acclimated to greenhouse conditions and developed flowers within 4 weeks of transplanting.

AB - Aristolochia fimbriata is a small herbaceous perennial in the basal angiosperm family Aristolochiaceae. The family contains diverse floral forms ranging from radial to monosymmetric flowers with a wide variety of insect pollinators. Additionally, Aristolochia species contain secondary metabolites that are important natural toxins and traditional medicines, and are critical to the reproduction of swallowtail butterflies. These characteristics, in combination with the small genome size and short life cycle of A. fimbriata, have prompted further development of this species as a model system to study the evolution of basal angiosperms. As a prerequisite for developing a genetic transformation procedure for Aristolochia, we developed protocols for in vitro plant multiplication, shoot organogenesis, rooting, and acclimation of tissue culture-derived plants. Two varieties of Aristolochia were multiplied in vitro and rooted with 100% efficiency. Shoot regeneration was achieved within 1 month of culture initiation from whole leaf, internodal stem, and petiole explants. The highest regeneration success (97%) was recorded for stem explants. Regenerated and rooted shoots were acclimated to greenhouse conditions and developed flowers within 4 weeks of transplanting.

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