Regional activation in the rat brain during visceral stimulation detected by c-fos expression and fMRI

J. Lazovic, H. F. Wrzos, Q. X. Yang, C. M. Collins, M. B. Smith, R. Norgren, K. Matyas, Ann Ouyang

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Aim: The aim of the study was to determine and compare the areas of brain activated in response to colorectal distention (CRD) using functional magnetic resonance imaging (fMRI) and c-fos protein expression. Methods: For fMRI study (3.0 T magnet), anaesthetized rats underwent phasic CRD, synchronized with fMRI acquisition. Stimulation consisted of eight cycles of balloon deflation (90 s) and inflation (30 s), at 40, 60 or 80 mmHg of pressure. For c-fos study two sets of experiments were performed on anaesthetized rats: comparing (A) brain activation in rats with the inserted colorectal balloon (n = 5), to the rats without the balloon (n = 5); and (B) rats with inserted balloon (n = 10), to the rats with inserted and distended balloon (n = 10). The pressure of 80 mmHg was applied for 2 h of 30 s inflation and 90 s deflation, alternating cycles. Results: Functional MRI revealed significant activation in the amygdala, hypothalamus, thalamus, cerebellum and hippocampus. Significant increase in c-fos expression was observed in amygdala and thalamus in the first set of experiments, and hypothalamus and parabrachial nuclei in the second. Conclusion: The two methods are not interchangeable but appeared to be complementary: fMRI was more sensitive, whereas c-fos had much greater resolution.

Original languageEnglish (US)
Pages (from-to)548-556
Number of pages9
JournalNeurogastroenterology and Motility
Volume17
Issue number4
DOIs
StatePublished - Aug 1 2005

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Magnetic Resonance Imaging
Brain
Economic Inflation
Amygdala
Thalamus
Hypothalamus
Proto-Oncogene Proteins c-fos
Pressure
Magnets
Cerebellum
Hippocampus

All Science Journal Classification (ASJC) codes

  • Physiology
  • Endocrine and Autonomic Systems
  • Gastroenterology

Cite this

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title = "Regional activation in the rat brain during visceral stimulation detected by c-fos expression and fMRI",
abstract = "Aim: The aim of the study was to determine and compare the areas of brain activated in response to colorectal distention (CRD) using functional magnetic resonance imaging (fMRI) and c-fos protein expression. Methods: For fMRI study (3.0 T magnet), anaesthetized rats underwent phasic CRD, synchronized with fMRI acquisition. Stimulation consisted of eight cycles of balloon deflation (90 s) and inflation (30 s), at 40, 60 or 80 mmHg of pressure. For c-fos study two sets of experiments were performed on anaesthetized rats: comparing (A) brain activation in rats with the inserted colorectal balloon (n = 5), to the rats without the balloon (n = 5); and (B) rats with inserted balloon (n = 10), to the rats with inserted and distended balloon (n = 10). The pressure of 80 mmHg was applied for 2 h of 30 s inflation and 90 s deflation, alternating cycles. Results: Functional MRI revealed significant activation in the amygdala, hypothalamus, thalamus, cerebellum and hippocampus. Significant increase in c-fos expression was observed in amygdala and thalamus in the first set of experiments, and hypothalamus and parabrachial nuclei in the second. Conclusion: The two methods are not interchangeable but appeared to be complementary: fMRI was more sensitive, whereas c-fos had much greater resolution.",
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Regional activation in the rat brain during visceral stimulation detected by c-fos expression and fMRI. / Lazovic, J.; Wrzos, H. F.; Yang, Q. X.; Collins, C. M.; Smith, M. B.; Norgren, R.; Matyas, K.; Ouyang, Ann.

In: Neurogastroenterology and Motility, Vol. 17, No. 4, 01.08.2005, p. 548-556.

Research output: Contribution to journalArticle

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AU - Lazovic, J.

AU - Wrzos, H. F.

AU - Yang, Q. X.

AU - Collins, C. M.

AU - Smith, M. B.

AU - Norgren, R.

AU - Matyas, K.

AU - Ouyang, Ann

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