Regional brain iron, transferrin receptor and transferrin levels in iron deficient anemic rats

J. L. Beard, N. Q. Li, D. J. Pinero, K. Erikson, James Connor

Research output: Contribution to journalArticle

Abstract

This study sought to gain understanding of the effect of iron deficiency anemia (IDA) on regional brain iron, transferrin receptor (TfR), and transferrin (Tf) in the developing rodent brain. Male weanling Sprague-Dawley rats were used for this study. One group (n=10) received AIN-76A control diet with 35 ppm Fe (CN) and the second group the same diet without iron (<5 ppm Fe) for 28 days. Anemia was verified in the IDA group via hemoglobin (Hb)=5.3 g/dL. After sacrifice, brains and livers were rapidly removed and the brains dissected into eight regions. Each region was pooled and analyzed by tissue ELISA or immunoblot for TfR and Tf. Iron analysis was performed by atomic absorption spectrophotometry. As noted previous studies, regional iron levels were significantly lower (p<0.05) in IDA compared to CN with significant variation by brain region. TfR and Tf responses to IDA were most pronounced in cortex, a region that did not have a large drop in iron content. Other regions like cerebellum, striatum, and thalamus had no response to IDA. None of the responses were as pronounced as in liver. These data indicate a heterogeneous response to IDA by the brain with respect to brain iron, TfR, and Tf levels and suggest that some regions may use mechanisms other than the Tf-TfR route for regulating iron movement.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998

Fingerprint

Transferrin Receptors
transferrin
Transferrin
Iron-Deficiency Anemias
Rats
Brain
Iron
iron deficiency anemia
iron
brain
receptors
rats
Diet
Atomic Spectrophotometry
Nutrition
Liver
Iron analysis
Thalamus
Cerebellum
Sprague Dawley Rats

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Beard, J. L. ; Li, N. Q. ; Pinero, D. J. ; Erikson, K. ; Connor, James. / Regional brain iron, transferrin receptor and transferrin levels in iron deficient anemic rats. In: FASEB Journal. 1998 ; Vol. 12, No. 5.
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Regional brain iron, transferrin receptor and transferrin levels in iron deficient anemic rats. / Beard, J. L.; Li, N. Q.; Pinero, D. J.; Erikson, K.; Connor, James.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

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