Regional Cerebral Glucose Utilization During Insulin‐Induced Hypoglycemia in Unanesthetized Rats

Robert M. Bryan, Kerry A. Keefer, Colin MacNeill

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


Regional cerebral glucose utilization (rCMRgl) was studied during insulin‐induced hypoglycemia in unanesthetized rats. Rats were surgically prepared using halothane and nitrous oxide anesthesia and allowed 5 h to recover from the anesthesia before rCMRgl was measured. The rCMRgl was measured using [6‐14C]glucose in a normoglycemic control group and two hypoglycemic groups, A (30 min after insulin injection) and B (2 h after insulin injection). The mean plasma glucose level was 7.03 μmol/ml in the normoglycemic group, 1.96 μmol/ml in hypoglycemic group A, and 1.40 μmol/ml in hypoglycemic group B. The rCMRgl in hypoglycemic group A decreased 8–18% in 17 brain regions measured; five changes were statistically significant. The rCMRgl in hypoglycemic group B decreased significantly in all but one of the brain regions measured; the decrease ranged from 15% in the pyramidal tract to 36% in the motor and auditory cortices. The rCMRgl in every brain region decreased when the plasma glucose level fell below 1.5–2.5 μmol/ml. No brain region could maintain rCMRgl at plasma glucose concentrations lower than predicted by regional glucose influx described in previous studies. Glucose utilization in all brain regions appears to be limited by the influx of glucose.

Original languageEnglish (US)
Pages (from-to)1904-1911
Number of pages8
JournalJournal of neurochemistry
Issue number6
StatePublished - Jun 1986

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience


Dive into the research topics of 'Regional Cerebral Glucose Utilization During Insulin‐Induced Hypoglycemia in Unanesthetized Rats'. Together they form a unique fingerprint.

Cite this