Regional interlaboratory standardization of determinations of cholesterol, high-density lipoprotein cholesterol, and triglycerides

G. A. Tetrault, W. G. Miller, Vernon Chinchilli, B. Brown, T. Balby, P. Rooney, S. Bennett, V. Seckman, M. Dickens, J. Johnson, D. Ligon, B. Hull, R. Carpenter, W. Steinmetz, L. Mason, K. Freude, C. St. Charles, B. Huffington, A. M. Rosendale

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The Clinical Chemistry Forum of Central Virginia initiated a lipid standardization program to help ensure that its members meet the current National Cholesterol Education Program guidelines for cholesterol testing, and to standardize assays of high-density lipoprotein (HDL) cholesterol and triglycerides so as to provide accurate lipid profiles. We found that freshly collected, never-frozen human sera must be used to assess interlaboratory accuracy for cholesterol, HDL cholesterol, andd triglycerides assays, and that at least 23 samples are required to detect a 3% bias with 90% power when the between-laboratory imprecision (CV) is 3%. After recalibration, all 12 laboratories had a mean cholesterol bias ≤5%, nine of 10 laboratories had a mean HDL cholesterol bias ≤40 mg/L for samples with values ≤570 mg/L, and 10 of 12 laboratories had a mean triglycerides bias ≤10% for fresh human sera split between participants and the Centers for Disease Control. Pools of frozen human serum were shown to have matrix biases >3% for cholesterol in seven of 11 laboratories, and >40 mg/L for HDL cholesterol in six of nine laboratories.

Original languageEnglish (US)
Pages (from-to)145-149
Number of pages5
JournalClinical Chemistry
Volume36
Issue number1
StatePublished - Feb 19 1990

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Cholesterol
Standardization
HDL Cholesterol
Assays
Triglycerides
Serum
Disease control
Lipids
Clinical Chemistry
Centers for Disease Control and Prevention (U.S.)
lipoprotein triglyceride
Education
Guidelines
Testing

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Tetrault, G. A., Miller, W. G., Chinchilli, V., Brown, B., Balby, T., Rooney, P., ... Rosendale, A. M. (1990). Regional interlaboratory standardization of determinations of cholesterol, high-density lipoprotein cholesterol, and triglycerides. Clinical Chemistry, 36(1), 145-149.
Tetrault, G. A. ; Miller, W. G. ; Chinchilli, Vernon ; Brown, B. ; Balby, T. ; Rooney, P. ; Bennett, S. ; Seckman, V. ; Dickens, M. ; Johnson, J. ; Ligon, D. ; Hull, B. ; Carpenter, R. ; Steinmetz, W. ; Mason, L. ; Freude, K. ; St. Charles, C. ; Huffington, B. ; Rosendale, A. M. / Regional interlaboratory standardization of determinations of cholesterol, high-density lipoprotein cholesterol, and triglycerides. In: Clinical Chemistry. 1990 ; Vol. 36, No. 1. pp. 145-149.
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abstract = "The Clinical Chemistry Forum of Central Virginia initiated a lipid standardization program to help ensure that its members meet the current National Cholesterol Education Program guidelines for cholesterol testing, and to standardize assays of high-density lipoprotein (HDL) cholesterol and triglycerides so as to provide accurate lipid profiles. We found that freshly collected, never-frozen human sera must be used to assess interlaboratory accuracy for cholesterol, HDL cholesterol, andd triglycerides assays, and that at least 23 samples are required to detect a 3{\%} bias with 90{\%} power when the between-laboratory imprecision (CV) is 3{\%}. After recalibration, all 12 laboratories had a mean cholesterol bias ≤5{\%}, nine of 10 laboratories had a mean HDL cholesterol bias ≤40 mg/L for samples with values ≤570 mg/L, and 10 of 12 laboratories had a mean triglycerides bias ≤10{\%} for fresh human sera split between participants and the Centers for Disease Control. Pools of frozen human serum were shown to have matrix biases >3{\%} for cholesterol in seven of 11 laboratories, and >40 mg/L for HDL cholesterol in six of nine laboratories.",
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Tetrault, GA, Miller, WG, Chinchilli, V, Brown, B, Balby, T, Rooney, P, Bennett, S, Seckman, V, Dickens, M, Johnson, J, Ligon, D, Hull, B, Carpenter, R, Steinmetz, W, Mason, L, Freude, K, St. Charles, C, Huffington, B & Rosendale, AM 1990, 'Regional interlaboratory standardization of determinations of cholesterol, high-density lipoprotein cholesterol, and triglycerides', Clinical Chemistry, vol. 36, no. 1, pp. 145-149.

Regional interlaboratory standardization of determinations of cholesterol, high-density lipoprotein cholesterol, and triglycerides. / Tetrault, G. A.; Miller, W. G.; Chinchilli, Vernon; Brown, B.; Balby, T.; Rooney, P.; Bennett, S.; Seckman, V.; Dickens, M.; Johnson, J.; Ligon, D.; Hull, B.; Carpenter, R.; Steinmetz, W.; Mason, L.; Freude, K.; St. Charles, C.; Huffington, B.; Rosendale, A. M.

In: Clinical Chemistry, Vol. 36, No. 1, 19.02.1990, p. 145-149.

Research output: Contribution to journalArticle

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T1 - Regional interlaboratory standardization of determinations of cholesterol, high-density lipoprotein cholesterol, and triglycerides

AU - Tetrault, G. A.

AU - Miller, W. G.

AU - Chinchilli, Vernon

AU - Brown, B.

AU - Balby, T.

AU - Rooney, P.

AU - Bennett, S.

AU - Seckman, V.

AU - Dickens, M.

AU - Johnson, J.

AU - Ligon, D.

AU - Hull, B.

AU - Carpenter, R.

AU - Steinmetz, W.

AU - Mason, L.

AU - Freude, K.

AU - St. Charles, C.

AU - Huffington, B.

AU - Rosendale, A. M.

PY - 1990/2/19

Y1 - 1990/2/19

N2 - The Clinical Chemistry Forum of Central Virginia initiated a lipid standardization program to help ensure that its members meet the current National Cholesterol Education Program guidelines for cholesterol testing, and to standardize assays of high-density lipoprotein (HDL) cholesterol and triglycerides so as to provide accurate lipid profiles. We found that freshly collected, never-frozen human sera must be used to assess interlaboratory accuracy for cholesterol, HDL cholesterol, andd triglycerides assays, and that at least 23 samples are required to detect a 3% bias with 90% power when the between-laboratory imprecision (CV) is 3%. After recalibration, all 12 laboratories had a mean cholesterol bias ≤5%, nine of 10 laboratories had a mean HDL cholesterol bias ≤40 mg/L for samples with values ≤570 mg/L, and 10 of 12 laboratories had a mean triglycerides bias ≤10% for fresh human sera split between participants and the Centers for Disease Control. Pools of frozen human serum were shown to have matrix biases >3% for cholesterol in seven of 11 laboratories, and >40 mg/L for HDL cholesterol in six of nine laboratories.

AB - The Clinical Chemistry Forum of Central Virginia initiated a lipid standardization program to help ensure that its members meet the current National Cholesterol Education Program guidelines for cholesterol testing, and to standardize assays of high-density lipoprotein (HDL) cholesterol and triglycerides so as to provide accurate lipid profiles. We found that freshly collected, never-frozen human sera must be used to assess interlaboratory accuracy for cholesterol, HDL cholesterol, andd triglycerides assays, and that at least 23 samples are required to detect a 3% bias with 90% power when the between-laboratory imprecision (CV) is 3%. After recalibration, all 12 laboratories had a mean cholesterol bias ≤5%, nine of 10 laboratories had a mean HDL cholesterol bias ≤40 mg/L for samples with values ≤570 mg/L, and 10 of 12 laboratories had a mean triglycerides bias ≤10% for fresh human sera split between participants and the Centers for Disease Control. Pools of frozen human serum were shown to have matrix biases >3% for cholesterol in seven of 11 laboratories, and >40 mg/L for HDL cholesterol in six of nine laboratories.

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