Regulation and action of the bacterial enhancer-binding protein AAA+ domains

Baoyu Chen, Tatyana A. Sysoeva, Saikat Chowdhury, B. Tracy Nixon

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Bacterial EBPs (enhancer-binding proteins) play crucial roles in regulating cellular responses to environmental changes, in part by providing efficient control over σ54-dependent gene transcription. The AAA+ (ATPase associated with various cellular activites) domain of the EBPs, when assembled into a ring, uses energy from ATP binding, hydrolysis and product release to remodel the σ54-RNAP (RNA polymerase) holoenzyme so that it can transition from closed to open form at promoter DNA. The assembly, and hence activity, of these ATPases are regulated by many different signal transduction mechanisms. Recent advances in solution scattering techniques, when combined with high-resolution structures and biochemical data, have enabled us to obtain mechanistic insights into the regulation and action of a subset of these σ54 activators: those whose assembly into ring form is controlled by two-component signal transduction. We review (i) experimental considerations of applying the SAXS (small-angle X-ray scattering)/WAXS (wide-angle X-ray scattering) technique, (ii) distinct regulation mechanisms of the AAA+ domains of three EBPs by similar two-component signal transduction receiver domains, and (iii) major conformational changes and correlated σ54-binding activity of an isolated EBP AAA+ domain in the ATP hydrolysis cycle.

Original languageEnglish (US)
Pages (from-to)89-93
Number of pages5
JournalBiochemical Society transactions
Volume36
Issue number1
DOIs
StatePublished - Feb 1 2008

All Science Journal Classification (ASJC) codes

  • Biochemistry

Fingerprint Dive into the research topics of 'Regulation and action of the bacterial enhancer-binding protein AAA+ domains'. Together they form a unique fingerprint.

  • Cite this