Regulation of a virus-induced lethal disease in tomato revealed by LongSAGE analysis

Saeed Irian, Ping Xu, Xinbin Dai, Patrick X. Zhao, Marilyn J. Roossinck

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Infection of Cucumber mosaic virus (CMV) and D satellite RNA (satRNA) in tomato plants induces rapid plant death, which has caused catastrophic crop losses. We conducted long serial analysis of gene expression (LongSAGE) in control and virus-infected plants to identify the genes that may be involved in the development of this lethal tomato disease. The transcriptomes were compared between mock-inoculated plants and plants infected with CMV, CMV/D satRNA, or CMV/Dm satRNA (a nonnecrogenic mutant of D satRNA with three mutated nucleotides). The analysis revealed both general and specific changes in the tomato transcriptome after infection with these viruses. A massive transcriptional difference of approximately 400 genes was found between the transcriptomes of CMV/D and CMV/Dm satRNA-infected plants. Particularly, the Long-SAGE data indicated the activation of ethylene synthesis and signaling by CMV/D satRNA infection. Results from inoculation tests with an ethylene-insensitive mutant and treatments with an ethylene action inhibitor further confirmed the role of ethylene in mediating the epinastic leaf symptoms and the secondary cell death in the stem. Results from Northern blot analysis demonstrated the partial contribution of ethylene in the induced defense responses in CMV/D satRNA-infected plants.

Original languageEnglish (US)
Pages (from-to)1477-1488
Number of pages12
JournalMolecular Plant-Microbe Interactions
Volume20
Issue number12
DOIs
StatePublished - Dec 1 2007

Fingerprint

Cucumber Mosaic Virus Satellite
satellite RNA
Cucumber mosaic virus
Lycopersicon esculentum
lethal genes
tomatoes
Viruses
Gene Expression
gene expression
viruses
ethylene
Transcriptome
Cucumovirus
transcriptome
Satellite RNAs
Plant Viruses
infection
Virus Diseases
Infection
Northern Blotting

All Science Journal Classification (ASJC) codes

  • Physiology
  • Agronomy and Crop Science

Cite this

Irian, Saeed ; Xu, Ping ; Dai, Xinbin ; Zhao, Patrick X. ; Roossinck, Marilyn J. / Regulation of a virus-induced lethal disease in tomato revealed by LongSAGE analysis. In: Molecular Plant-Microbe Interactions. 2007 ; Vol. 20, No. 12. pp. 1477-1488.
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Regulation of a virus-induced lethal disease in tomato revealed by LongSAGE analysis. / Irian, Saeed; Xu, Ping; Dai, Xinbin; Zhao, Patrick X.; Roossinck, Marilyn J.

In: Molecular Plant-Microbe Interactions, Vol. 20, No. 12, 01.12.2007, p. 1477-1488.

Research output: Contribution to journalArticle

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