Regulation of cell-to-cell variability in divergent gene expression

Chao Yan, Shuyang Wu, Christopher Pocetti, Lu Bai

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Cell-to-cell variability (noise) is an important feature of gene expression that impacts cell fitness and development. The regulatory mechanism of this variability is not fully understood. Here we investigate the effect on gene expression noise in divergent gene pairs (DGPs). We generated reporters driven by divergent promoters, rearranged their gene order, and probed their expressions using time-lapse fluorescence microscopy and single-molecule fluorescence in situ hybridization (smFISH). We show that two genes in a co-regulated DGP have higher expression covariance compared with the separate, tandem and convergent configurations, and this higher covariance is caused by more synchronized firing of the divergent transcriptions. For differentially regulated DGPs, the regulatory signal of one gene can stochastically "leak"™ to the other, causing increased gene expression noise. We propose that the DGPs"™ function in limiting or promoting gene expression noise may enhance or compromise cell fitness, providing an explanation for the conservation pattern of DGPs.

Original languageEnglish (US)
Article number11099
JournalNature communications
Volume7
DOIs
StatePublished - Mar 24 2016

Fingerprint

gene expression
Gene expression
genes
Genes
Cells
Gene Expression
Noise
cells
fitness
Gene Order
Regulator Genes
Fluorescence In Situ Hybridization
Fluorescence Microscopy
fluorescence
Fluorescence microscopy
Transcription
conservation
Conservation
Fluorescence
microscopy

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Yan, Chao ; Wu, Shuyang ; Pocetti, Christopher ; Bai, Lu. / Regulation of cell-to-cell variability in divergent gene expression. In: Nature communications. 2016 ; Vol. 7.
@article{e87578b6a9de4667a53772798efa69d0,
title = "Regulation of cell-to-cell variability in divergent gene expression",
abstract = "Cell-to-cell variability (noise) is an important feature of gene expression that impacts cell fitness and development. The regulatory mechanism of this variability is not fully understood. Here we investigate the effect on gene expression noise in divergent gene pairs (DGPs). We generated reporters driven by divergent promoters, rearranged their gene order, and probed their expressions using time-lapse fluorescence microscopy and single-molecule fluorescence in situ hybridization (smFISH). We show that two genes in a co-regulated DGP have higher expression covariance compared with the separate, tandem and convergent configurations, and this higher covariance is caused by more synchronized firing of the divergent transcriptions. For differentially regulated DGPs, the regulatory signal of one gene can stochastically {"}leak{"}™ to the other, causing increased gene expression noise. We propose that the DGPs{"}™ function in limiting or promoting gene expression noise may enhance or compromise cell fitness, providing an explanation for the conservation pattern of DGPs.",
author = "Chao Yan and Shuyang Wu and Christopher Pocetti and Lu Bai",
year = "2016",
month = "3",
day = "24",
doi = "10.1038/ncomms11099",
language = "English (US)",
volume = "7",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",

}

Regulation of cell-to-cell variability in divergent gene expression. / Yan, Chao; Wu, Shuyang; Pocetti, Christopher; Bai, Lu.

In: Nature communications, Vol. 7, 11099, 24.03.2016.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Regulation of cell-to-cell variability in divergent gene expression

AU - Yan, Chao

AU - Wu, Shuyang

AU - Pocetti, Christopher

AU - Bai, Lu

PY - 2016/3/24

Y1 - 2016/3/24

N2 - Cell-to-cell variability (noise) is an important feature of gene expression that impacts cell fitness and development. The regulatory mechanism of this variability is not fully understood. Here we investigate the effect on gene expression noise in divergent gene pairs (DGPs). We generated reporters driven by divergent promoters, rearranged their gene order, and probed their expressions using time-lapse fluorescence microscopy and single-molecule fluorescence in situ hybridization (smFISH). We show that two genes in a co-regulated DGP have higher expression covariance compared with the separate, tandem and convergent configurations, and this higher covariance is caused by more synchronized firing of the divergent transcriptions. For differentially regulated DGPs, the regulatory signal of one gene can stochastically "leak"™ to the other, causing increased gene expression noise. We propose that the DGPs"™ function in limiting or promoting gene expression noise may enhance or compromise cell fitness, providing an explanation for the conservation pattern of DGPs.

AB - Cell-to-cell variability (noise) is an important feature of gene expression that impacts cell fitness and development. The regulatory mechanism of this variability is not fully understood. Here we investigate the effect on gene expression noise in divergent gene pairs (DGPs). We generated reporters driven by divergent promoters, rearranged their gene order, and probed their expressions using time-lapse fluorescence microscopy and single-molecule fluorescence in situ hybridization (smFISH). We show that two genes in a co-regulated DGP have higher expression covariance compared with the separate, tandem and convergent configurations, and this higher covariance is caused by more synchronized firing of the divergent transcriptions. For differentially regulated DGPs, the regulatory signal of one gene can stochastically "leak"™ to the other, causing increased gene expression noise. We propose that the DGPs"™ function in limiting or promoting gene expression noise may enhance or compromise cell fitness, providing an explanation for the conservation pattern of DGPs.

UR - http://www.scopus.com/inward/record.url?scp=84962321313&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84962321313&partnerID=8YFLogxK

U2 - 10.1038/ncomms11099

DO - 10.1038/ncomms11099

M3 - Article

VL - 7

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 11099

ER -