Regulation of CXC chemokine receptor 4-mediated migration by the Tec family tyrosine kinase ITK

Angela M. Fischer, Jason C. Mercer, Archana Iyer, Melanie J. Ragin, Avery August

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Chemokines are critical in controlling lymphocyte traffic and migration. The CXC chemokine CXCL12/SDF-1α interacts with its receptor CXCR4 to induce the migration of a number of different cell types. Although an understanding of the physiological functions of this chemokine is emerging, the mechanism by which it regulates T cell migration is still unclear. We show here that the Tec family kinase ITK is activated rapidly following CXCL12/SDF-1α stimulation, and this requires Src and phosphatidylinositol 3-kinase activities. ITK regulates the ability of CXCL12/SDF-1α to induce T cell migration as overexpression of wild-type ITK-enhanced migration, and T cells lacking ITK exhibit reduced migration as well as adhesion in response to CXCL12/SDF-1α. Further analysis suggests that ITK may regulate CXCR4-mediated migration and adhesion by altering the actin cytoskeleton, as ITK null T cells were significantly defective in CXCL12/SDF-1α-mediated actin polymerization. Our data suggest that ITK may regulate the ability of CXCR4 to induce T cell migration.

Original languageEnglish (US)
Pages (from-to)29816-29820
Number of pages5
JournalJournal of Biological Chemistry
Volume279
Issue number28
DOIs
StatePublished - Jul 9 2004

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CXCR4 Receptors
T-cells
Protein-Tyrosine Kinases
T-Lymphocytes
Cell Movement
Chemokines
Actins
Adhesion
Phosphatidylinositol 3-Kinase
Chemokine CXCL12
CXC Chemokines
Null Lymphocytes
Lymphocytes
Actin Cytoskeleton
Polymerization
Phosphotransferases
Cell Count
Tec protein-tyrosine kinase

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Fischer, Angela M. ; Mercer, Jason C. ; Iyer, Archana ; Ragin, Melanie J. ; August, Avery. / Regulation of CXC chemokine receptor 4-mediated migration by the Tec family tyrosine kinase ITK. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 28. pp. 29816-29820.
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Regulation of CXC chemokine receptor 4-mediated migration by the Tec family tyrosine kinase ITK. / Fischer, Angela M.; Mercer, Jason C.; Iyer, Archana; Ragin, Melanie J.; August, Avery.

In: Journal of Biological Chemistry, Vol. 279, No. 28, 09.07.2004, p. 29816-29820.

Research output: Contribution to journalArticle

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