Regulation of DLG localization at synapses by CaMKII-dependent phosphorylation

Young Ho Koh, Evgenya Popova, Ulrich Thomas, Leslie C. Griffith, Vivian Budnik

Research output: Contribution to journalArticle

189 Citations (Scopus)

Abstract

Discs large (DLG) mediates the clustering of synaptic molecules. Here we demonstrate that synaptic localization of DLG itself is regulated by CaMKII. We show that DLG and CaMKII colocalize at synapses and exist in the same protein complex. Constitutively activated CaMKII phenocopied structural abnormalities of dig mutant synapses and dramatically increased extrajunctional DLG. Decreased CaMKII activity caused opposite alterations. In vitro, CaMKII phosphorylated a DLG fragment with a stoichiometry close to one. Moreover, expression of site-directed dig mutants that blocked or mimicked phosphorylation had effects similar to those observed upon inhibiting or constitutively activating CaMKII. We propose that CaMKII- dependent DLG phosphorylation regulates the association of DLG with the synaptic complex during development and plasticity, thus providing a link between synaptic activity and structure.

Original languageEnglish (US)
Pages (from-to)353-363
Number of pages11
JournalCell
Volume98
Issue number3
DOIs
StatePublished - Aug 6 1999

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Calcium-Calmodulin-Dependent Protein Kinase Type 2
Phosphorylation
Synapses
Stoichiometry
Plasticity
Cluster Analysis
Association reactions
Molecules

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Koh, Young Ho ; Popova, Evgenya ; Thomas, Ulrich ; Griffith, Leslie C. ; Budnik, Vivian. / Regulation of DLG localization at synapses by CaMKII-dependent phosphorylation. In: Cell. 1999 ; Vol. 98, No. 3. pp. 353-363.
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Regulation of DLG localization at synapses by CaMKII-dependent phosphorylation. / Koh, Young Ho; Popova, Evgenya; Thomas, Ulrich; Griffith, Leslie C.; Budnik, Vivian.

In: Cell, Vol. 98, No. 3, 06.08.1999, p. 353-363.

Research output: Contribution to journalArticle

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