Regulation of factors controlling angiogenesis in liver development: A role for PEDF in the formation and maintenance of normal vasculature

S. Sawant, S. Aparicio, A. R. Tink, N. Lara, C. J. Barnstable, J. Tombran-Tink

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

PEDF and VEGF are important inhibitors and promoters of angiogenesis, and the ratio between the two is an important indicator in many neovascular diseases. In mouse liver PEDF and VEGF 165 were co-expressed at very early stages of liver development and their expression increased as liver embryogenesis progressed, suggesting that PEDF and VEGF are both crucial to vasculogenesis as well. VEGF 189 only appears at the P0 stage in liver organogenesis and is maintained at high levels thereafter. PEDF and the two VEGF isoforms are synthesized by fresh and cultured hepatocytes. Expression of VEGF 121 and overexpression of VEGF 165 were only seen in HepG2, a well-characterized hepatocellular carcinoma line. The results suggest that hepatic vascular architecture is under the control of both PEDF and VEGF, and that VEGF 165 and VEGF 189 have distinct functions in normal vascular development of the liver. The VEGF isoforms 121 and 189 may be key regulators of increased vascularity and progression of hepatocellular carcinoma, one of the most common malignant tumors, and may be of prognostic significance for this tumor.

Original languageEnglish (US)
Pages (from-to)408-413
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume325
Issue number2
DOIs
StatePublished - Dec 10 2004

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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