Regulation of inflammatory and lipid metabolism genes by eicosapentaenoic acid-rich oil

Peter J. Gillies, Sujata K. Bhatia, Leigh A. Belcher, Daniel B. Hannon, Jerry T. Thompson, John Patrick Vanden Heuvel

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Omega-3-PUFAs, eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA), are associated with prevention of various aspects of metabolic syndrome. In the present studies, the effects of oil rich in EPA on gene expression and activation of nuclear receptors was examined and compared with other ω3-PUFAs. The EPA-rich oil (EO) altered the expression of FA metabolism genes in THP-1 cells, including stearoyl CoA desaturase (SCD) and FA desaturase- 1 and -2 (FASDS1 and -2). Other ω3-PUFAs resulted in a similar gene expression response for a subset of genes involved in lipid metabolism and inflammation. In reporter assays, EO activated human peroxisome proliferator-activated receptor α (PPARα) and PPARβ/γ with minimal effects on PPARγ, liver X receptor, retinoid X receptor, farnesoid X receptor, and retinoid acid receptor γ (RARγ); these effects were similar to that observed for purified EPA. When serum from a 6 week clinical intervention with dietary supplements containing olive oil (control), DHA, or two levels of EPA were applied to THP-1 cells, the expression of SCD and FADS2 decreased in the cells treated with serum from the ω3-PUFA- supplemented individuals. Taken together, these studies indicate regulation of gene expression by EO that is consistent with treating aspects of dyslipidemia and inflammation.

Original languageEnglish (US)
Pages (from-to)1679-1689
Number of pages11
JournalJournal of Lipid Research
Volume53
Issue number8
DOIs
StatePublished - Aug 1 2012

Fingerprint

Eicosapentaenoic Acid
Lipid Metabolism
Oils
Genes
Peroxisome Proliferator-Activated Receptors
Stearoyl-CoA Desaturase
Gene expression
Retinoid X Receptors
Docosahexaenoic Acids
Dietary supplements
Inflammation
Gene Expression
Gene Expression Regulation
Cytoplasmic and Nuclear Receptors
Dyslipidemias
Dietary Supplements
Serum
Metabolism
Liver
Transcriptional Activation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

Gillies, Peter J. ; Bhatia, Sujata K. ; Belcher, Leigh A. ; Hannon, Daniel B. ; Thompson, Jerry T. ; Vanden Heuvel, John Patrick. / Regulation of inflammatory and lipid metabolism genes by eicosapentaenoic acid-rich oil. In: Journal of Lipid Research. 2012 ; Vol. 53, No. 8. pp. 1679-1689.
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Regulation of inflammatory and lipid metabolism genes by eicosapentaenoic acid-rich oil. / Gillies, Peter J.; Bhatia, Sujata K.; Belcher, Leigh A.; Hannon, Daniel B.; Thompson, Jerry T.; Vanden Heuvel, John Patrick.

In: Journal of Lipid Research, Vol. 53, No. 8, 01.08.2012, p. 1679-1689.

Research output: Contribution to journalArticle

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