Regulation of nucleosome landscape and transcription factor targeting at tissue-specific enhancers by BRG1

Gangqing Hu, Dustin E. Schones, Kairong Cui, River Ybarra, Daniel Northrup, Qingsong Tang, Luca Gattinoni, Nicholas P. Restifo, Suming Huang, Keji Zhao

Research output: Contribution to journalArticle

112 Scopus citations

Abstract

Enhancers of transcription activate transcription via binding of sequence-specific transcription factors to their target sites in chromatin. In this report, we identify GATA1-bound distal sites genome-wide and find a global reorganization of the nucleosomes at these potential enhancers during differentiation of hematopoietic stem cells (HSCs) to erythrocytes. We show that the catalytic subunit BRG1 of BAF complexes localizes to these distal sites during differentiation and generates a longer nucleosome linker region surrounding the GATA1 sites by shifting the flanking nucleosomes away. Intriguingly, we find that the nucleosome shifting specifically facilitates binding of TAL1 but not GATA1 and is linked to subsequent transcriptional regulation of target genes.

Original languageEnglish (US)
Pages (from-to)1650-1658
Number of pages9
JournalGenome research
Volume21
Issue number10
DOIs
StatePublished - Oct 1 2011

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

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    Hu, G., Schones, D. E., Cui, K., Ybarra, R., Northrup, D., Tang, Q., Gattinoni, L., Restifo, N. P., Huang, S., & Zhao, K. (2011). Regulation of nucleosome landscape and transcription factor targeting at tissue-specific enhancers by BRG1. Genome research, 21(10), 1650-1658. https://doi.org/10.1101/gr.121145.111