Regulation of nucleosome landscape and transcription factor targeting at tissue-specific enhancers by BRG1

Gangqing Hu, Dustin E. Schones, Kairong Cui, River Ybarra, Daniel Northrup, Qingsong Tang, Luca Gattinoni, Nicholas P. Restifo, Suming Huang, Keji Zhao

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

Enhancers of transcription activate transcription via binding of sequence-specific transcription factors to their target sites in chromatin. In this report, we identify GATA1-bound distal sites genome-wide and find a global reorganization of the nucleosomes at these potential enhancers during differentiation of hematopoietic stem cells (HSCs) to erythrocytes. We show that the catalytic subunit BRG1 of BAF complexes localizes to these distal sites during differentiation and generates a longer nucleosome linker region surrounding the GATA1 sites by shifting the flanking nucleosomes away. Intriguingly, we find that the nucleosome shifting specifically facilitates binding of TAL1 but not GATA1 and is linked to subsequent transcriptional regulation of target genes.

Original languageEnglish (US)
Pages (from-to)1650-1658
Number of pages9
JournalGenome Research
Volume21
Issue number10
DOIs
StatePublished - Oct 1 2011

Fingerprint

Nucleosomes
Transcription Factors
Hematopoietic Stem Cells
Chromatin
Catalytic Domain
Erythrocytes
Genome
Genes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

Cite this

Hu, G., Schones, D. E., Cui, K., Ybarra, R., Northrup, D., Tang, Q., ... Zhao, K. (2011). Regulation of nucleosome landscape and transcription factor targeting at tissue-specific enhancers by BRG1. Genome Research, 21(10), 1650-1658. https://doi.org/10.1101/gr.121145.111
Hu, Gangqing ; Schones, Dustin E. ; Cui, Kairong ; Ybarra, River ; Northrup, Daniel ; Tang, Qingsong ; Gattinoni, Luca ; Restifo, Nicholas P. ; Huang, Suming ; Zhao, Keji. / Regulation of nucleosome landscape and transcription factor targeting at tissue-specific enhancers by BRG1. In: Genome Research. 2011 ; Vol. 21, No. 10. pp. 1650-1658.
@article{05787449982146dfa6bdcffb1537b25b,
title = "Regulation of nucleosome landscape and transcription factor targeting at tissue-specific enhancers by BRG1",
abstract = "Enhancers of transcription activate transcription via binding of sequence-specific transcription factors to their target sites in chromatin. In this report, we identify GATA1-bound distal sites genome-wide and find a global reorganization of the nucleosomes at these potential enhancers during differentiation of hematopoietic stem cells (HSCs) to erythrocytes. We show that the catalytic subunit BRG1 of BAF complexes localizes to these distal sites during differentiation and generates a longer nucleosome linker region surrounding the GATA1 sites by shifting the flanking nucleosomes away. Intriguingly, we find that the nucleosome shifting specifically facilitates binding of TAL1 but not GATA1 and is linked to subsequent transcriptional regulation of target genes.",
author = "Gangqing Hu and Schones, {Dustin E.} and Kairong Cui and River Ybarra and Daniel Northrup and Qingsong Tang and Luca Gattinoni and Restifo, {Nicholas P.} and Suming Huang and Keji Zhao",
year = "2011",
month = "10",
day = "1",
doi = "10.1101/gr.121145.111",
language = "English (US)",
volume = "21",
pages = "1650--1658",
journal = "Genome Research",
issn = "1088-9051",
publisher = "Cold Spring Harbor Laboratory Press",
number = "10",

}

Hu, G, Schones, DE, Cui, K, Ybarra, R, Northrup, D, Tang, Q, Gattinoni, L, Restifo, NP, Huang, S & Zhao, K 2011, 'Regulation of nucleosome landscape and transcription factor targeting at tissue-specific enhancers by BRG1', Genome Research, vol. 21, no. 10, pp. 1650-1658. https://doi.org/10.1101/gr.121145.111

Regulation of nucleosome landscape and transcription factor targeting at tissue-specific enhancers by BRG1. / Hu, Gangqing; Schones, Dustin E.; Cui, Kairong; Ybarra, River; Northrup, Daniel; Tang, Qingsong; Gattinoni, Luca; Restifo, Nicholas P.; Huang, Suming; Zhao, Keji.

In: Genome Research, Vol. 21, No. 10, 01.10.2011, p. 1650-1658.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Regulation of nucleosome landscape and transcription factor targeting at tissue-specific enhancers by BRG1

AU - Hu, Gangqing

AU - Schones, Dustin E.

AU - Cui, Kairong

AU - Ybarra, River

AU - Northrup, Daniel

AU - Tang, Qingsong

AU - Gattinoni, Luca

AU - Restifo, Nicholas P.

AU - Huang, Suming

AU - Zhao, Keji

PY - 2011/10/1

Y1 - 2011/10/1

N2 - Enhancers of transcription activate transcription via binding of sequence-specific transcription factors to their target sites in chromatin. In this report, we identify GATA1-bound distal sites genome-wide and find a global reorganization of the nucleosomes at these potential enhancers during differentiation of hematopoietic stem cells (HSCs) to erythrocytes. We show that the catalytic subunit BRG1 of BAF complexes localizes to these distal sites during differentiation and generates a longer nucleosome linker region surrounding the GATA1 sites by shifting the flanking nucleosomes away. Intriguingly, we find that the nucleosome shifting specifically facilitates binding of TAL1 but not GATA1 and is linked to subsequent transcriptional regulation of target genes.

AB - Enhancers of transcription activate transcription via binding of sequence-specific transcription factors to their target sites in chromatin. In this report, we identify GATA1-bound distal sites genome-wide and find a global reorganization of the nucleosomes at these potential enhancers during differentiation of hematopoietic stem cells (HSCs) to erythrocytes. We show that the catalytic subunit BRG1 of BAF complexes localizes to these distal sites during differentiation and generates a longer nucleosome linker region surrounding the GATA1 sites by shifting the flanking nucleosomes away. Intriguingly, we find that the nucleosome shifting specifically facilitates binding of TAL1 but not GATA1 and is linked to subsequent transcriptional regulation of target genes.

UR - http://www.scopus.com/inward/record.url?scp=80053501181&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80053501181&partnerID=8YFLogxK

U2 - 10.1101/gr.121145.111

DO - 10.1101/gr.121145.111

M3 - Article

VL - 21

SP - 1650

EP - 1658

JO - Genome Research

JF - Genome Research

SN - 1088-9051

IS - 10

ER -