Regulation of rat brain/hepg2 glucose transporter gene expression by insulin and insulin-like growth factor-i in primary cultures of neuronal and glial cells

Haim Werner, Howard L. Foyt, Charles T. Roberts, Derek Leroith, Ian Simpson, Mohan K. Raizada, Laura M. Mudd

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

We have demonstrated the expression of the rat brain/HepG2 glucose transporter gene in primary cultures of rat neuronal and glial cells by Northern blot analysis with a rat brain glucose transporter cDNA probe. Incubation of both neuronal and glial cells with insulin and insulin-like growth factor- I induced a time- and dose-dependent increase in the steady state levels of glucose transporter mRNA. The maximal response was achieved between 2â€4 h and subsequently decreased. Both insulin and insulin-like growth factor-I at a dose of 1 ng/ml elicited an approximately 57% increase in glucose transporter mRNA levels in neuronal cultures after 90 min, suggesting that each peptide was acting through its own receptor. On the other hand, insulin stimulated [3H]2-deoxyglucose uptake in glial, but not neuronal, cells. These results suggest that insulin-like peptides regulate the expression of the rat brain/Hep G2 glucose transporter gene at both transcriptional and posttranscriptional levels, and that these regulatory mechanisms may be dissociated from each other. Insulin-like peptides may, therefore, participate in the control of brain energy metabolism.

Original languageEnglish (US)
Pages (from-to)314-320
Number of pages7
JournalEndocrinology
Volume125
Issue number1
DOIs
StatePublished - Jan 1 1989

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Facilitative Glucose Transport Proteins
Somatomedins
Neuroglia
Insulin
Gene Expression
Brain
Insulin-Like Growth Factor I
Peptides
Messenger RNA
Deoxyglucose
Northern Blotting
Energy Metabolism
Genes
Complementary DNA

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

Werner, Haim ; Foyt, Howard L. ; Roberts, Charles T. ; Leroith, Derek ; Simpson, Ian ; Raizada, Mohan K. ; Mudd, Laura M. / Regulation of rat brain/hepg2 glucose transporter gene expression by insulin and insulin-like growth factor-i in primary cultures of neuronal and glial cells. In: Endocrinology. 1989 ; Vol. 125, No. 1. pp. 314-320.
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Regulation of rat brain/hepg2 glucose transporter gene expression by insulin and insulin-like growth factor-i in primary cultures of neuronal and glial cells. / Werner, Haim; Foyt, Howard L.; Roberts, Charles T.; Leroith, Derek; Simpson, Ian; Raizada, Mohan K.; Mudd, Laura M.

In: Endocrinology, Vol. 125, No. 1, 01.01.1989, p. 314-320.

Research output: Contribution to journalArticle

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