Regulation of the activity of 3-hydroxy-3-methylglutaryl coenzyme a reductase by insulin

Clare Sample, Gene C. Ness

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

In hypophysectomized - diabetic rats full restoration of hepatic HMG-CoA reductase activity required administration of both triiodothyronine and insulin. Giving triiodothyronine alone resulted in full restoration of reductase mRNA and immunoreactive protein but not of enzyme activity. Increasing the ratio of dithiothreitol to microsomal protein resulted in full HMG-CoA reductase activity. With 5mM glutathione, HMG-CoA reductase activity was 5 to 10 fold higher in non-diabetic animals. The data suggest that insulin acts by increasing the portion of HMG-CoA reductase present in its active free sulfhydryl form.

Original languageEnglish (US)
Pages (from-to)201-207
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume137
Issue number1
DOIs
StatePublished - May 29 1986

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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