Regulation of the transcriptional activator NtrC1: Structural studies of the regulatory and AAA+ ATPase domains

Seok Yong Lee, Armando De La Torre, Dalai Yan, Sydney Kustu, B. Tracy Nixon, David E. Wemmer

Research output: Contribution to journalArticle

156 Citations (Scopus)

Abstract

Transcription by σ54 RNA polymerase depends on activators that contain ATPase domains of the AAA+ class. These activators, which are often response regulators of two-component signal transduction systems, remodel the polymerase so that it can form open complexes at promoters. Here, we report the first crystal structures of the ATPase domain of an activator, the NtrC1 protein from the extreme thermophile Aquifex aeolicus. This domain alone, which is active, crystallized as a ring-shaped heptamer. The protein carrying both the ATPase and adjacent receiver domains, which is inactive, crystallized as a dimer. In the inactive dimer, one residue needed for catalysis is far from the active site, and extensive contacts among the domains prevent oligomerization of the ATPase domain. Oligomerization, which completes the active site, depends on surfaces that are buried in the dimer, and hence, on a rearrangement of the receiver domains upon phosphorylation. A motif in the ATPase domain known to be critical for coupling energy to remodeling of polymerase forms a novel loop that projects from the middle of an α helix. The extended, structured loops from the subunits of the heptamer localize to a pore in the center of the ring and form a surface that could contact σ54.

Original languageEnglish (US)
Pages (from-to)2552-2563
Number of pages12
JournalGenes and Development
Volume17
Issue number20
DOIs
StatePublished - Oct 15 2003

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Adenosine Triphosphatases
Catalytic Domain
DNA-Directed RNA Polymerases
Catalysis
Signal Transduction
Proteins
Phosphorylation

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Cite this

Lee, Seok Yong ; De La Torre, Armando ; Yan, Dalai ; Kustu, Sydney ; Nixon, B. Tracy ; Wemmer, David E. / Regulation of the transcriptional activator NtrC1 : Structural studies of the regulatory and AAA+ ATPase domains. In: Genes and Development. 2003 ; Vol. 17, No. 20. pp. 2552-2563.
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Regulation of the transcriptional activator NtrC1 : Structural studies of the regulatory and AAA+ ATPase domains. / Lee, Seok Yong; De La Torre, Armando; Yan, Dalai; Kustu, Sydney; Nixon, B. Tracy; Wemmer, David E.

In: Genes and Development, Vol. 17, No. 20, 15.10.2003, p. 2552-2563.

Research output: Contribution to journalArticle

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