Relationship between expression of apoptosis-modulating proteins and multidrug resistance in KS62/VCR cells

Yusheng Zhu, Yuan Lu, Guo Wei Lin, Rong Xing Zhang, Zheng Hong Yang, Yuan Gang Lu

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: To explore the Relationship between expression of apoptosis-modulating proteins and multidrug resistance in K562/VCR cells. Methods: Immunocytochemical method and western blot were used to analyze the expression of apoptosis-modulating proteins (Bcl - 2, Bcl-XL, Bax, Bak) in multidrug resistant cell line K562/VCR and drugsensitive cell line K562. Results: The positive cell rates of apoptosis-suppressing protein Bcl-2 and Bcl-XL in K562/VCR were (40.0 ± 8.0) % and (60.0 ± 10.0). While the rates in K562 were (1.0 ± 0.3)% and (20.0 ± 4.0)%. There was significant difference in the positive cell rates of Bcl - 2 and Bcl - XL between K562/VCR and K562 (n = 3, P < 0.05). It was also found there was no significant difference in expression of Bax between K562/VCR and K562. Furthermore, Bak was not expressed in both K562/VCR and K562 or the expression was very low. Conclusions: We suggest that Bcl-2 and Bcl-XL play important roles in multidrug resistance in K562/VCR, while Bax and Bak might not be important.

Original languageEnglish (US)
Pages (from-to)32-34
Number of pages3
JournalFudan University Journal of Medical Sciences
Volume28
Issue number1
StatePublished - Dec 1 2001

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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