Relationship between expression of apoptosis-modulating proteins and multidrug resistance in K562/VCR cells

Yusheng Zhu, Y. Lu, G. W. Lin, R. X. Zhang, Z. H. Yang, Y. G. Lu

Research output: Contribution to journalArticle

Abstract

Purpose To explore the Relationship between expression of apoptosis-modulating proteins and multidrug resistance in K562/VCR cells. Methods Immunocytochemical method and western blot were used to analyze the expression of apoptosis-modulating proteins(Bcl - 2, Bcl-XL, Bax, Bak) in multidrug resistant cell line K562/VCR and drugsensitive cell line K562. Results The positive cell rates of apoptosis-suppressing protein Bcl-2 and Bcl-XL in K562/VCR were (40.0 ± 8.0) % and (60.0 ± 10.0) %. While the rates in K562 were (1.0 ± 0.3) % and (20.0 ± 4.0)%. There was significant difference in the positive cell rates of Bcl - 2 and Bcl - XL between K562/VCR and K562(n = 3, P < 0.05). It was also found there was no significant difference in expression of Bax between K562/VCR and K562. Furthermore, Bak was not expressed in both K562/VCR and K562 or the expression was very low. Conclusions We suggest that Bcl-2 and Bcl-XL play important roles in multidrug resistance in K562/VCR, while Bax and Bak might not be important.

Original languageEnglish (US)
Pages (from-to)32-34
Number of pages3
JournalJournal of Shanghai Medical University
Volume28
Issue number1
StatePublished - Jan 1 2001

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P-Glycoproteins
K562 Cells
Apoptosis
Cell Line
Multiple Drug Resistance
Proteins
Western Blotting

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Zhu, Yusheng ; Lu, Y. ; Lin, G. W. ; Zhang, R. X. ; Yang, Z. H. ; Lu, Y. G. / Relationship between expression of apoptosis-modulating proteins and multidrug resistance in K562/VCR cells. In: Journal of Shanghai Medical University. 2001 ; Vol. 28, No. 1. pp. 32-34.
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abstract = "Purpose To explore the Relationship between expression of apoptosis-modulating proteins and multidrug resistance in K562/VCR cells. Methods Immunocytochemical method and western blot were used to analyze the expression of apoptosis-modulating proteins(Bcl - 2, Bcl-XL, Bax, Bak) in multidrug resistant cell line K562/VCR and drugsensitive cell line K562. Results The positive cell rates of apoptosis-suppressing protein Bcl-2 and Bcl-XL in K562/VCR were (40.0 ± 8.0) {\%} and (60.0 ± 10.0) {\%}. While the rates in K562 were (1.0 ± 0.3) {\%} and (20.0 ± 4.0){\%}. There was significant difference in the positive cell rates of Bcl - 2 and Bcl - XL between K562/VCR and K562(n = 3, P < 0.05). It was also found there was no significant difference in expression of Bax between K562/VCR and K562. Furthermore, Bak was not expressed in both K562/VCR and K562 or the expression was very low. Conclusions We suggest that Bcl-2 and Bcl-XL play important roles in multidrug resistance in K562/VCR, while Bax and Bak might not be important.",
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Relationship between expression of apoptosis-modulating proteins and multidrug resistance in K562/VCR cells. / Zhu, Yusheng; Lu, Y.; Lin, G. W.; Zhang, R. X.; Yang, Z. H.; Lu, Y. G.

In: Journal of Shanghai Medical University, Vol. 28, No. 1, 01.01.2001, p. 32-34.

Research output: Contribution to journalArticle

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AU - Lu, Y.

AU - Lin, G. W.

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AU - Yang, Z. H.

AU - Lu, Y. G.

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AB - Purpose To explore the Relationship between expression of apoptosis-modulating proteins and multidrug resistance in K562/VCR cells. Methods Immunocytochemical method and western blot were used to analyze the expression of apoptosis-modulating proteins(Bcl - 2, Bcl-XL, Bax, Bak) in multidrug resistant cell line K562/VCR and drugsensitive cell line K562. Results The positive cell rates of apoptosis-suppressing protein Bcl-2 and Bcl-XL in K562/VCR were (40.0 ± 8.0) % and (60.0 ± 10.0) %. While the rates in K562 were (1.0 ± 0.3) % and (20.0 ± 4.0)%. There was significant difference in the positive cell rates of Bcl - 2 and Bcl - XL between K562/VCR and K562(n = 3, P < 0.05). It was also found there was no significant difference in expression of Bax between K562/VCR and K562. Furthermore, Bak was not expressed in both K562/VCR and K562 or the expression was very low. Conclusions We suggest that Bcl-2 and Bcl-XL play important roles in multidrug resistance in K562/VCR, while Bax and Bak might not be important.

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