Relationship between p53 mutation incidence in oral cavity squamous cell carcinomas and patient tobacco use

Philip Lazarus, Jordan Stern, Neil Zwiebel, Alex Fair, John Richie, Stimson Schantz

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

It is well-established that a high incidence of p53 mutations exist in oral cavity squamous cell carcinomas (OCSCCs). To determine whether p53 mutations are etiologically associated with OCSCC development or are associated with exposure to specific carcinogens, we have analyzed the conserved regions of the p53 gene (exons 5-9) in 48 OCSCCs obtained from patients with varied tobacco and alcohol use histories by polymerase chain reaction/single strand conformational polymorphism (PCR/SSCP) and DNA sequencing analysis. Thirty-eight percent (18/48) of the OCSCCs exhibited a mutation in exons 5-9 of the p53 gene. There was a significantly higher incidence of p53 mutations in OCSCCs from tobacco users (predominantly cigarette smokers) compared to those who had never used tobacco. No increase in the incidence of p53 mutation was observed in tobacco users who drank alcohol. G to A transitions and deletions were the predominant mutations observed in OCSCCs from tobacco users. No specific pattern of mutation was observed in OCSCCs from those subjects who had never used tobacco. These data suggest that a history of tobacco use was associated with a high incidence of p53 mutations in patients with OCSCC and that tobacco carcinogens induce a specific pattern of mutations in oral cavity tissue in vivo.

Original languageEnglish (US)
Pages (from-to)733-739
Number of pages7
JournalCarcinogenesis
Volume17
Issue number4
DOIs
StatePublished - Apr 1 1996

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Tobacco Use
Mouth
Squamous Cell Carcinoma
Mutation
Tobacco
Incidence
p53 Genes
Carcinogens
Exons
Alcohols
DNA Sequence Analysis
Tobacco Products
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Cancer Research

Cite this

Lazarus, Philip ; Stern, Jordan ; Zwiebel, Neil ; Fair, Alex ; Richie, John ; Schantz, Stimson. / Relationship between p53 mutation incidence in oral cavity squamous cell carcinomas and patient tobacco use. In: Carcinogenesis. 1996 ; Vol. 17, No. 4. pp. 733-739.
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Relationship between p53 mutation incidence in oral cavity squamous cell carcinomas and patient tobacco use. / Lazarus, Philip; Stern, Jordan; Zwiebel, Neil; Fair, Alex; Richie, John; Schantz, Stimson.

In: Carcinogenesis, Vol. 17, No. 4, 01.04.1996, p. 733-739.

Research output: Contribution to journalArticle

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