Relative abundance of tsetse fly species and their infection rates in simanjiro, Northern Tanzania

L. P. Salekwa, H. J. Nnko, A. Ngonyoka, Anna Bond Estes, M. Agaba, P. S. Gwakisa

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

This study was conducted to determine the prevalence of trypanosomes, infective to cattle and humans in tsetse flies caught in Simanjiro district, which borders with Tarangire National park in northern Tanzania. A total of 1000 tsetse flies were caught during the semi dry season in July and August 2014 using F 3 and Epsilon traps. Results revealed Glossina swynertonni as the most abundant species (72%) followed by Glossina morsitans morsitans (21%) and Glossina pallidipes (7%) in the study area. Whole fly DNA was extracted from all the 1000 flies caught in the study area.

Analysis of fly DNA for detection of trypanosome species showed a 3% (30/1000) overall infection rate of which 13.3% (4/30) flies were co-infected with Trypanosoma brucei and Trypanosoma vivax, while 3.3% (1/30) were co-infected with Trypanosoma congolense and Tripanosoma vivax. Majority of flies (83.3%, 25/30) were infected with T. vivax. All three trypanosome species were detected in G.swynertonni flies but G.m. morsitans carried T. vivax and T. brucei. No parasites were detected in G. pallidipes flies. Further, no human infective trypanosomes were detected when T. brucei positive flies were analysed with specific primers for SRA-PCR. The study confirms presence of cattle infective trypanosomes and absence of human-infective trypanosomes in tsetse flies caught in the study area.

Original languageEnglish (US)
JournalLivestock Research for Rural Development
Volume26
Issue number12
StatePublished - Dec 1 2014

Fingerprint

Glossina
Tanzania
Trypanosoma vivax
Trypanosoma brucei
infection
Glossina pallidipes
Glossina morsitans morsitans
Trypanosoma congolense
cattle
DNA
dry season
national parks
traps
parasites

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

Cite this

Salekwa, L. P. ; Nnko, H. J. ; Ngonyoka, A. ; Estes, Anna Bond ; Agaba, M. ; Gwakisa, P. S. / Relative abundance of tsetse fly species and their infection rates in simanjiro, Northern Tanzania. In: Livestock Research for Rural Development. 2014 ; Vol. 26, No. 12.
@article{3bfeb1405248404caaba5abfb1975e34,
title = "Relative abundance of tsetse fly species and their infection rates in simanjiro, Northern Tanzania",
abstract = "This study was conducted to determine the prevalence of trypanosomes, infective to cattle and humans in tsetse flies caught in Simanjiro district, which borders with Tarangire National park in northern Tanzania. A total of 1000 tsetse flies were caught during the semi dry season in July and August 2014 using F 3 and Epsilon traps. Results revealed Glossina swynertonni as the most abundant species (72{\%}) followed by Glossina morsitans morsitans (21{\%}) and Glossina pallidipes (7{\%}) in the study area. Whole fly DNA was extracted from all the 1000 flies caught in the study area.Analysis of fly DNA for detection of trypanosome species showed a 3{\%} (30/1000) overall infection rate of which 13.3{\%} (4/30) flies were co-infected with Trypanosoma brucei and Trypanosoma vivax, while 3.3{\%} (1/30) were co-infected with Trypanosoma congolense and Tripanosoma vivax. Majority of flies (83.3{\%}, 25/30) were infected with T. vivax. All three trypanosome species were detected in G.swynertonni flies but G.m. morsitans carried T. vivax and T. brucei. No parasites were detected in G. pallidipes flies. Further, no human infective trypanosomes were detected when T. brucei positive flies were analysed with specific primers for SRA-PCR. The study confirms presence of cattle infective trypanosomes and absence of human-infective trypanosomes in tsetse flies caught in the study area.",
author = "Salekwa, {L. P.} and Nnko, {H. J.} and A. Ngonyoka and Estes, {Anna Bond} and M. Agaba and Gwakisa, {P. S.}",
year = "2014",
month = "12",
day = "1",
language = "English (US)",
volume = "26",
journal = "Livestock Research for Rural Development",
issn = "0121-3784",
publisher = "Centro para la Investigacion en Sistemas Sostenibles de Produccion Agropecuaria",
number = "12",

}

Relative abundance of tsetse fly species and their infection rates in simanjiro, Northern Tanzania. / Salekwa, L. P.; Nnko, H. J.; Ngonyoka, A.; Estes, Anna Bond; Agaba, M.; Gwakisa, P. S.

In: Livestock Research for Rural Development, Vol. 26, No. 12, 01.12.2014.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Relative abundance of tsetse fly species and their infection rates in simanjiro, Northern Tanzania

AU - Salekwa, L. P.

AU - Nnko, H. J.

AU - Ngonyoka, A.

AU - Estes, Anna Bond

AU - Agaba, M.

AU - Gwakisa, P. S.

PY - 2014/12/1

Y1 - 2014/12/1

N2 - This study was conducted to determine the prevalence of trypanosomes, infective to cattle and humans in tsetse flies caught in Simanjiro district, which borders with Tarangire National park in northern Tanzania. A total of 1000 tsetse flies were caught during the semi dry season in July and August 2014 using F 3 and Epsilon traps. Results revealed Glossina swynertonni as the most abundant species (72%) followed by Glossina morsitans morsitans (21%) and Glossina pallidipes (7%) in the study area. Whole fly DNA was extracted from all the 1000 flies caught in the study area.Analysis of fly DNA for detection of trypanosome species showed a 3% (30/1000) overall infection rate of which 13.3% (4/30) flies were co-infected with Trypanosoma brucei and Trypanosoma vivax, while 3.3% (1/30) were co-infected with Trypanosoma congolense and Tripanosoma vivax. Majority of flies (83.3%, 25/30) were infected with T. vivax. All three trypanosome species were detected in G.swynertonni flies but G.m. morsitans carried T. vivax and T. brucei. No parasites were detected in G. pallidipes flies. Further, no human infective trypanosomes were detected when T. brucei positive flies were analysed with specific primers for SRA-PCR. The study confirms presence of cattle infective trypanosomes and absence of human-infective trypanosomes in tsetse flies caught in the study area.

AB - This study was conducted to determine the prevalence of trypanosomes, infective to cattle and humans in tsetse flies caught in Simanjiro district, which borders with Tarangire National park in northern Tanzania. A total of 1000 tsetse flies were caught during the semi dry season in July and August 2014 using F 3 and Epsilon traps. Results revealed Glossina swynertonni as the most abundant species (72%) followed by Glossina morsitans morsitans (21%) and Glossina pallidipes (7%) in the study area. Whole fly DNA was extracted from all the 1000 flies caught in the study area.Analysis of fly DNA for detection of trypanosome species showed a 3% (30/1000) overall infection rate of which 13.3% (4/30) flies were co-infected with Trypanosoma brucei and Trypanosoma vivax, while 3.3% (1/30) were co-infected with Trypanosoma congolense and Tripanosoma vivax. Majority of flies (83.3%, 25/30) were infected with T. vivax. All three trypanosome species were detected in G.swynertonni flies but G.m. morsitans carried T. vivax and T. brucei. No parasites were detected in G. pallidipes flies. Further, no human infective trypanosomes were detected when T. brucei positive flies were analysed with specific primers for SRA-PCR. The study confirms presence of cattle infective trypanosomes and absence of human-infective trypanosomes in tsetse flies caught in the study area.

UR - http://www.scopus.com/inward/record.url?scp=84914145882&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84914145882&partnerID=8YFLogxK

M3 - Article

VL - 26

JO - Livestock Research for Rural Development

JF - Livestock Research for Rural Development

SN - 0121-3784

IS - 12

ER -