Release of nonstop ribosomes is essential

Heather A. Feaga, Patrick H. Viollier, Kenneth C. Keiler

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Bacterial ribosomes frequently translate to the 3= end of an mRNA without terminating at a stop codon. Almost all bacteria use the transfer-messenger RNA (tmRNA)-based trans-translation pathway to release these “nonstop” ribosomes and maintain protein synthesis capacity. trans-translation is essential in some species, but in others, such as Caulobacter crescentus, trans-translation can be inactivated. To determine why trans-translation is dispensable in C. crescentus, a Tn-seq screen was used to identify genes that specifically alter growth in cells lacking ssrA, the gene encoding tmRNA. One of these genes, CC1214, was essential in ΔssrA cells. Purified CC1214 protein could release nonstop ribosomes in vitro. CC1214 is a homolog of the Escherichia coli ArfB protein, and using the CC1214 sequence, ArfB homologs were identified in the majority of bacterial phyla. Most species in which ssrA has been deleted contain an ArfB homolog, suggesting that release of nonstop ribosomes may be essential in most or all bacteria.

Original languageEnglish (US)
Article numbere01916-14
JournalmBio
Volume5
Issue number6
DOIs
StatePublished - Sep 7 2014

Fingerprint

Ribosomes
Caulobacter crescentus
Transfer RNA
Messenger RNA
Genes
Bacteria
Terminator Codon
Escherichia coli Proteins
Sequence Homology
Proteins
Growth

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Virology

Cite this

Feaga, Heather A. ; Viollier, Patrick H. ; Keiler, Kenneth C. / Release of nonstop ribosomes is essential. In: mBio. 2014 ; Vol. 5, No. 6.
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Release of nonstop ribosomes is essential. / Feaga, Heather A.; Viollier, Patrick H.; Keiler, Kenneth C.

In: mBio, Vol. 5, No. 6, e01916-14, 07.09.2014.

Research output: Contribution to journalArticle

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