TY - JOUR
T1 - Remote Control of Neural Stem Cell Fate Using NIR-Responsive Photoswitching Upconversion Nanoparticle Constructs
AU - Zhang, Yixiao
AU - Wiesholler, Lisa M.
AU - Rabie, Hudifah
AU - Jiang, Pengfei
AU - Lai, Jinping
AU - Hirsch, Thomas
AU - Lee, Ki Bum
N1 - Funding Information:
K.-B.L. acknowledges partial financial support from the NIH R21 (1R21NS085569 and R21AR071101), NIH R01 (1R01DC016612-01 and 3R01DC016612-01S1), New Jersey Commission on Spinal Cord Research [CSCR17IRG010 and CSCR16ERG019], NSF [CHE-1429062 and CBET-1803517], and ACS New Directions Award (PRF# 55869-ND10). T.H. acknowledges Sandy Himmelstoß for the TEM studies.
Funding Information:
K.-B.L. acknowledges partial financial support from the NIH R21 (1R21NS085569 and R21AR071101) NIH R01 (1R01DC016612-01 and 3R01DC016612-01S1), New Jersey Commission on Spinal Cord Research [CSCR17IRG010 and CSCR16ERG019], NSF [CHE-1429062 and CBET-1803517], and ACS New Directions Award (PRF# 55869-ND10). T.H. acknowledges Sandy Himmelsto? for the TEM studies.
Publisher Copyright:
© 2020 American Chemical Society.
PY - 2020/9/9
Y1 - 2020/9/9
N2 - Light-mediated remote control of stem cell fate, such as proliferation, differentiation, and migration, can bring a significant impact on stem cell biology and regenerative medicine. Current UV/vis-mediated control approaches are limited in terms of nonspecific absorption, poor tissue penetration, and phototoxicity. Upconversion nanoparticle (UCNP)-based near-infrared (NIR)-mediated control systems have gained increasing attention for vast applications with minimal nonspecific absorption, good penetration depth, and minimal phototoxicity from NIR excitations. Specifically, 808 nm NIR-responsive upconversion nanomaterials have shown clear advantages for biomedical applications owing to diminished heating effects and better tissue penetration. Herein, a novel 808 nm NIR-mediated control method for stem cell differentiation has been developed using multishell UCNPs, which are optimized for upconverting 808 nm NIR light to UV emission. The locally generated UV emissions further toggle photoswitching polymer capping ligands to achieve spatiotemporally controlled small-molecule release. More specifically, with 808 nm NIR excitation, stem cell differentiation factors can be released to guide neural stem cell (NSC) differentiation in a highly controlled manner. Given the challenges in stem cell behavior control, the developed 808 nm NIR-responsive UCNP-based approach to control stem cell differentiation can represent a new tool for studying single-molecule roles in stem cell and developmental biology.
AB - Light-mediated remote control of stem cell fate, such as proliferation, differentiation, and migration, can bring a significant impact on stem cell biology and regenerative medicine. Current UV/vis-mediated control approaches are limited in terms of nonspecific absorption, poor tissue penetration, and phototoxicity. Upconversion nanoparticle (UCNP)-based near-infrared (NIR)-mediated control systems have gained increasing attention for vast applications with minimal nonspecific absorption, good penetration depth, and minimal phototoxicity from NIR excitations. Specifically, 808 nm NIR-responsive upconversion nanomaterials have shown clear advantages for biomedical applications owing to diminished heating effects and better tissue penetration. Herein, a novel 808 nm NIR-mediated control method for stem cell differentiation has been developed using multishell UCNPs, which are optimized for upconverting 808 nm NIR light to UV emission. The locally generated UV emissions further toggle photoswitching polymer capping ligands to achieve spatiotemporally controlled small-molecule release. More specifically, with 808 nm NIR excitation, stem cell differentiation factors can be released to guide neural stem cell (NSC) differentiation in a highly controlled manner. Given the challenges in stem cell behavior control, the developed 808 nm NIR-responsive UCNP-based approach to control stem cell differentiation can represent a new tool for studying single-molecule roles in stem cell and developmental biology.
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U2 - 10.1021/acsami.0c10145
DO - 10.1021/acsami.0c10145
M3 - Article
C2 - 32805826
AN - SCOPUS:85090870273
SN - 1944-8244
VL - 12
SP - 40031
EP - 40041
JO - ACS applied materials & interfaces
JF - ACS applied materials & interfaces
IS - 36
ER -