Repeat-induced point mutations in pad-1, a putative RNA splicing factor from Neurospora crassa, confer dominant lethal effects on ascus development

Gretchen Anna Kuldau, Namboori B. Raju, N. Louise Glass

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We describe the characterization of a gene, Pad-1, from Neurospora crassa which displays sequence characteristics of the RS class of hnRNA-binding proteins (hnRNP) and mRNA splicing factors. This is the first report of the isolation of a putative hnRNP gene from N. crassa. PAD-1 showed 30% identity and 57% similarity to a protein, HCC1, which was isolated using autoantibodies from patients suffering from hepatocellular carcinoma. Both HCC1 and PAD-1 show amino acid sequence similarities to the human splicing factor, U2AF65. Mutations induced in Pad-1 by repeat-induced point (RIP) mutation show dominant effects on ascus and ascospore formation, a novel phenotypic class of RIP mutants. A mutant isolated from the Pad-1 RIP cross displayed a severe vegetative growth defect and dominant effects on ascus development, indicating that Pad-1 is essential for both asexual and sexual development.

Original languageEnglish (US)
Pages (from-to)169-180
Number of pages12
JournalFungal Genetics and Biology
Volume23
Issue number2
DOIs
StatePublished - Jan 1 1998

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Neurospora crassa
Point Mutation
Carrier Proteins
Protein Splicing
Sexual Development
Autoantibodies
Genes
Amino Acid Sequence
Hepatocellular Carcinoma
Messenger RNA
Mutation
Growth
Proteins
RNA Splicing Factors

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Genetics

Cite this

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abstract = "We describe the characterization of a gene, Pad-1, from Neurospora crassa which displays sequence characteristics of the RS class of hnRNA-binding proteins (hnRNP) and mRNA splicing factors. This is the first report of the isolation of a putative hnRNP gene from N. crassa. PAD-1 showed 30{\%} identity and 57{\%} similarity to a protein, HCC1, which was isolated using autoantibodies from patients suffering from hepatocellular carcinoma. Both HCC1 and PAD-1 show amino acid sequence similarities to the human splicing factor, U2AF65. Mutations induced in Pad-1 by repeat-induced point (RIP) mutation show dominant effects on ascus and ascospore formation, a novel phenotypic class of RIP mutants. A mutant isolated from the Pad-1 RIP cross displayed a severe vegetative growth defect and dominant effects on ascus development, indicating that Pad-1 is essential for both asexual and sexual development.",
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Repeat-induced point mutations in pad-1, a putative RNA splicing factor from Neurospora crassa, confer dominant lethal effects on ascus development. / Kuldau, Gretchen Anna; Raju, Namboori B.; Glass, N. Louise.

In: Fungal Genetics and Biology, Vol. 23, No. 2, 01.01.1998, p. 169-180.

Research output: Contribution to journalArticle

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