Replacement of adenylate cyclase toxin in a lineage of Bordetella bronchiseptica

Anne M. Buboltz, Tracy L. Nicholson, Mylisa R. Parette, Sara E. Hester, Julian Parkhill, Eric T. Harvill

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Bordetella bronchiseptica is a gram-negative respiratory pathogen that infects a wide range of hosts and causes a diverse spectrum of disease. This diversity is likely affected by multiple factors, such as host immune status, polymicrobial infection, and strain diversity. In a murine model of infection, we found that the virulence of B. bronchiseptica strains, as measured by the mean lethal dose, varied widely. Strain 253 was less virulent than the typically studied strain, RB50. Transcriptome analysis showed that cyaA, the gene encoding adenylate cyclase toxin (CyaA), was the most downregulated transcript identified in strain 253 compared to that in strain RB50. Comparative genomic hybridization and genome sequencing of strain 253 revealed that the cya locus, which encodes, activates, and secretes CyaA, was replaced by an operon (ptp) predicted to encode peptide transport proteins. Other B. bronchiseptica strains from the same phylogenetic lineage as that of strain 253 also lacked the cya locus, contained the ptp genes, and were less virulent than strain RB50. Although the loss of CyaA would be expected to be counters elected since it is conserved among the classical bordetellae and believed to be important to their success, our data indicate that the loss of this toxin and the gain of the ptp genes occurred in an ancestral strain that then expanded into a lineage. This suggests that there may be ecological niches in which CyaA is not critical for the success of B. bronchiseptica.

Original languageEnglish (US)
Pages (from-to)5502-5511
Number of pages10
JournalJournal of bacteriology
Volume190
Issue number15
DOIs
StatePublished - Aug 1 2008

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Adenylate Cyclase Toxin
Bordetella bronchiseptica
Genes
Comparative Genomic Hybridization
Host Specificity
Gene Expression Profiling
Operon
Coinfection
Virulence
Carrier Proteins
Down-Regulation
Genome
Peptides
Infection

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Buboltz, A. M., Nicholson, T. L., Parette, M. R., Hester, S. E., Parkhill, J., & Harvill, E. T. (2008). Replacement of adenylate cyclase toxin in a lineage of Bordetella bronchiseptica. Journal of bacteriology, 190(15), 5502-5511. https://doi.org/10.1128/JB.00226-08
Buboltz, Anne M. ; Nicholson, Tracy L. ; Parette, Mylisa R. ; Hester, Sara E. ; Parkhill, Julian ; Harvill, Eric T. / Replacement of adenylate cyclase toxin in a lineage of Bordetella bronchiseptica. In: Journal of bacteriology. 2008 ; Vol. 190, No. 15. pp. 5502-5511.
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Buboltz, AM, Nicholson, TL, Parette, MR, Hester, SE, Parkhill, J & Harvill, ET 2008, 'Replacement of adenylate cyclase toxin in a lineage of Bordetella bronchiseptica', Journal of bacteriology, vol. 190, no. 15, pp. 5502-5511. https://doi.org/10.1128/JB.00226-08

Replacement of adenylate cyclase toxin in a lineage of Bordetella bronchiseptica. / Buboltz, Anne M.; Nicholson, Tracy L.; Parette, Mylisa R.; Hester, Sara E.; Parkhill, Julian; Harvill, Eric T.

In: Journal of bacteriology, Vol. 190, No. 15, 01.08.2008, p. 5502-5511.

Research output: Contribution to journalArticle

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Buboltz AM, Nicholson TL, Parette MR, Hester SE, Parkhill J, Harvill ET. Replacement of adenylate cyclase toxin in a lineage of Bordetella bronchiseptica. Journal of bacteriology. 2008 Aug 1;190(15):5502-5511. https://doi.org/10.1128/JB.00226-08