Replication clamps and clamp loaders

Mark Hedglin, Ravindra Kumar, Stephen J. Benkovic

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

To achieve the high degree of processivity required for DNA replication, DNA polymerases associate with ring-shaped sliding clamps that encircle the template DNA and slide freely along it. The closed circular structure of sliding clamps necessitates an enzyme-catalyzed mechanism, which not only opens them for assembly and closes them around DNA, but specifically targets them to sites where DNA synthesis is initiated and orients them correctly for replication. Such a feat is performed by multisubunit complexes known as clamp loaders, which use ATP to open sliding clamp rings and place them around the 3'end of primer- template (PT) junctions. Here we discuss the structure and composition of sliding clamps and clamp loaders from the three domains of life as well as T4 bacteriophage, and provide our current understanding of the clamp-loading process.

Original languageEnglish (US)
Pages (from-to)1-19
Number of pages19
JournalCold Spring Harbor Perspectives in Biology
Volume5
Issue number4
DOIs
StatePublished - Apr 1 2013

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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