Repository corticotropin injection reverses critical elements of the TLR9/B cell receptor activation response in human B cells in vitro

Nancy J. Olsen, Ann L. Benko, Carl A. McAloose, Patrice M. Becker, Dale Wright, Teresa Sunyer, Yuka Imamura Kawasawa, William J. Kovacs

Research output: Contribution to journalArticle

Abstract

We sought evidence for direct effects of repository corticotropin (RCI; an FDA-approved treatment for selected cases of SLE) on isolated human B lymphocytes activated by engagement of TLR9 and B cell receptors. ODN 2395/αIgM treatment was found to result in induction of 162 distinct mRNAs and suppression of 80 mRNAs at 24 h. RCI treatment resulted in suppression of 14 of the ODN 2395/αIgM -induced mRNAs (mean suppression to 23.6 ± 3.1% of stimulated value). The RCI-suppressed mRNAs included two critical regulators of class switch recombination, AICDA and BATF. RCI treatment also resulted in induction of 5 of the ODN 2395/αIgM -suppressed mRNAs (mean induction by RCI = 7.65 ± 2.34-fold). The RCI-induced mRNAs included SLAMF3, a cell surface receptor capable of inhibiting autoantibody responses. These studies reveal that RCI treatment of human B cells reverses key elements of the early mRNA response to TLR9 and B cell receptor engagement.

Original languageEnglish (US)
Pages (from-to)70-78
Number of pages9
JournalClinical Immunology
Volume201
DOIs
StatePublished - Apr 2019

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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