Repression of the Staphylococcus aureus accessory gene regulator in serum and in vivo

Jeremy M. Yarwood, John K. McCormick, Michael L. Paustian, Vivek Kapur, Patrick M. Schlievert

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Subgenomic DNA microarrays were employed to evaluate the expression of the accessory gene regulator (agr locus) as well as multiple virulence-associated genes in Staphylococcus aureus. Gene expression was examined during growth of S. aureus in vitro in standard laboratory medium and rabbit serum and in vivo in subcutaneous chambers implanted in either nonimmune rabbits or rabbits immunized with staphylococcal enterotoxin B. Expression of RNAIII, the effector molecule of the agr locus, was dramatically repressed in serum and in vivo, despite the increased expression of secreted virulence factors sufficient to cause toxic shock syndrome (TSS) in the animals. Statistical analysis and clustering of virulence genes based on their expression profiles in the various experimental conditions demonstrated no positive correlation between the expression of agr and any staphylococcal virulence factors examined. Disruption of the agr locus had only a minimal effect on the expression in vivo of the virulence factors examined. An effect of immunization on the expression of agr and virulence factors was also observed. These results suggest that agr activation is not necessary for development of staphylococcal TSS and that regulatory circuits responding to the in vivo environment override agr activity.

Original languageEnglish (US)
Pages (from-to)1095-1101
Number of pages7
JournalJournal of bacteriology
Volume184
Issue number4
DOIs
StatePublished - Feb 11 2002

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Virulence Factors
Regulator Genes
Staphylococcus aureus
Septic Shock
Rabbits
Serum
Virulence
Gene Expression
Oligonucleotide Array Sequence Analysis
Genes
Cluster Analysis
Immunization
Growth

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Yarwood, Jeremy M. ; McCormick, John K. ; Paustian, Michael L. ; Kapur, Vivek ; Schlievert, Patrick M. / Repression of the Staphylococcus aureus accessory gene regulator in serum and in vivo. In: Journal of bacteriology. 2002 ; Vol. 184, No. 4. pp. 1095-1101.
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Repression of the Staphylococcus aureus accessory gene regulator in serum and in vivo. / Yarwood, Jeremy M.; McCormick, John K.; Paustian, Michael L.; Kapur, Vivek; Schlievert, Patrick M.

In: Journal of bacteriology, Vol. 184, No. 4, 11.02.2002, p. 1095-1101.

Research output: Contribution to journalArticle

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