Reproduction and fertility in wild-type and transgenic mice after orthotopic transplantation of cryopreserved ovaries from 10-d-old mice

Li Jun Liu, Xia Yang Xie, Rui Zhong Zhang, Ping Xu, Hermann Bujard, Jun Miao

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Ovary cryopreservation and subsequent transplantation can enable researchers to preserve valuable transgenic animal strains. Some studies have indicated, however, that this process may impair ovary viability and recipient fertility. The authors investigated the effects of ovary vitrification followed by orthotopic transplantation in five strains of mice. They grafted fresh and frozen ovaries of 10-d-old mice into 4-week-old ovariectomized recipients. In addition to using wild-type strains (BALB/cAn and ICR/JCL), the authors used a transgene system that enabled them to identify whether offspring derived from the ovary of the recipient or that of the donor: they transplanted ovaries from one transgenic strain (LAP/rtTA) into wild-type C57BL/6J mice and into mice from a second transgenic strain (pTet/Cd226). The authors then determined the origin of the offspring born to these recipients using PCR. Ovary cryopreservation seemed to have no effect on the long-term fertility and reproductive characteristics of recipients and their offspring.

Original languageEnglish (US)
Pages (from-to)353-357
Number of pages5
JournalLab Animal
Volume37
Issue number8
DOIs
StatePublished - Aug 1 2008

Fingerprint

Transgenic Mice
Reproduction
Fertility
Ovary
Transplantation
genetically modified organisms
mice
cryopreservation
Cryopreservation
transgenic animals
vitrification
cans
Vitrification
transgenes
preserves
Genetically Modified Animals
researchers
viability
Transgenes
Inbred C57BL Mouse

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Liu, Li Jun ; Xie, Xia Yang ; Zhang, Rui Zhong ; Xu, Ping ; Bujard, Hermann ; Miao, Jun. / Reproduction and fertility in wild-type and transgenic mice after orthotopic transplantation of cryopreserved ovaries from 10-d-old mice. In: Lab Animal. 2008 ; Vol. 37, No. 8. pp. 353-357.
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Reproduction and fertility in wild-type and transgenic mice after orthotopic transplantation of cryopreserved ovaries from 10-d-old mice. / Liu, Li Jun; Xie, Xia Yang; Zhang, Rui Zhong; Xu, Ping; Bujard, Hermann; Miao, Jun.

In: Lab Animal, Vol. 37, No. 8, 01.08.2008, p. 353-357.

Research output: Contribution to journalArticle

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