Reptin52 expression during in vitro neural differentiation of human embryonic stem cells

Miguel Barthéléry, Amritha Jaishankar, Ugur Salli, Kent E. Vrana

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Human embryonic stem cells (hESCs) give rise to all somatic cell types, including neural cells such as astrocytes, oligodendrocytes and neurons. Commitment of hESC to a neural fate can be achieved via selection and expansion of developing neural stem cells, which, grown into non-adhering colonies called neurospheres, express nestin, a neurofilament marker. Analysis of hESC and hESC-derived neural stem cell nuclear extracts revealed an increased expression of Reptin52 in neurosphere nuclei. The increase in Reptin52 was evident throughout directed neuronal differentiation as assessed by western blotting, quantitative RT-PCR and immunocytochemistry. Reptin52 serves a pivotal regulatory role in nuclear activities such as transcription regulation and histone modification. In that regard, co-immunoprecipitation experiments showed that binding partners of Reptin52 (Pontin52, β-catenin and ATF-2) associate with this regulatory protein in hESC-derived neuronal precursors. Moreover, expression of two of these proteins (β-catenin - the end product of the Wnt signaling pathway - and ATF-2) is coordinately regulated with Reptin52.

Original languageEnglish (US)
Pages (from-to)47-51
Number of pages5
JournalNeuroscience letters
Volume452
Issue number1
DOIs
StatePublished - Mar 6 2009

Fingerprint

Catenins
Neural Stem Cells
Histone Code
Nestin
Wnt Signaling Pathway
Intermediate Filaments
Oligodendroglia
Cell Extracts
Immunoprecipitation
Astrocytes
Western Blotting
Immunohistochemistry
In Vitro Techniques
Human Embryonic Stem Cells
Neurons
Polymerase Chain Reaction
Proteins

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Barthéléry, Miguel ; Jaishankar, Amritha ; Salli, Ugur ; Vrana, Kent E. / Reptin52 expression during in vitro neural differentiation of human embryonic stem cells. In: Neuroscience letters. 2009 ; Vol. 452, No. 1. pp. 47-51.
@article{c6a79aac5c3b48f88631779e1078ad3d,
title = "Reptin52 expression during in vitro neural differentiation of human embryonic stem cells",
abstract = "Human embryonic stem cells (hESCs) give rise to all somatic cell types, including neural cells such as astrocytes, oligodendrocytes and neurons. Commitment of hESC to a neural fate can be achieved via selection and expansion of developing neural stem cells, which, grown into non-adhering colonies called neurospheres, express nestin, a neurofilament marker. Analysis of hESC and hESC-derived neural stem cell nuclear extracts revealed an increased expression of Reptin52 in neurosphere nuclei. The increase in Reptin52 was evident throughout directed neuronal differentiation as assessed by western blotting, quantitative RT-PCR and immunocytochemistry. Reptin52 serves a pivotal regulatory role in nuclear activities such as transcription regulation and histone modification. In that regard, co-immunoprecipitation experiments showed that binding partners of Reptin52 (Pontin52, β-catenin and ATF-2) associate with this regulatory protein in hESC-derived neuronal precursors. Moreover, expression of two of these proteins (β-catenin - the end product of the Wnt signaling pathway - and ATF-2) is coordinately regulated with Reptin52.",
author = "Miguel Barth{\'e}l{\'e}ry and Amritha Jaishankar and Ugur Salli and Vrana, {Kent E.}",
year = "2009",
month = "3",
day = "6",
doi = "10.1016/j.neulet.2009.01.023",
language = "English (US)",
volume = "452",
pages = "47--51",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

Reptin52 expression during in vitro neural differentiation of human embryonic stem cells. / Barthéléry, Miguel; Jaishankar, Amritha; Salli, Ugur; Vrana, Kent E.

In: Neuroscience letters, Vol. 452, No. 1, 06.03.2009, p. 47-51.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Reptin52 expression during in vitro neural differentiation of human embryonic stem cells

AU - Barthéléry, Miguel

AU - Jaishankar, Amritha

AU - Salli, Ugur

AU - Vrana, Kent E.

PY - 2009/3/6

Y1 - 2009/3/6

N2 - Human embryonic stem cells (hESCs) give rise to all somatic cell types, including neural cells such as astrocytes, oligodendrocytes and neurons. Commitment of hESC to a neural fate can be achieved via selection and expansion of developing neural stem cells, which, grown into non-adhering colonies called neurospheres, express nestin, a neurofilament marker. Analysis of hESC and hESC-derived neural stem cell nuclear extracts revealed an increased expression of Reptin52 in neurosphere nuclei. The increase in Reptin52 was evident throughout directed neuronal differentiation as assessed by western blotting, quantitative RT-PCR and immunocytochemistry. Reptin52 serves a pivotal regulatory role in nuclear activities such as transcription regulation and histone modification. In that regard, co-immunoprecipitation experiments showed that binding partners of Reptin52 (Pontin52, β-catenin and ATF-2) associate with this regulatory protein in hESC-derived neuronal precursors. Moreover, expression of two of these proteins (β-catenin - the end product of the Wnt signaling pathway - and ATF-2) is coordinately regulated with Reptin52.

AB - Human embryonic stem cells (hESCs) give rise to all somatic cell types, including neural cells such as astrocytes, oligodendrocytes and neurons. Commitment of hESC to a neural fate can be achieved via selection and expansion of developing neural stem cells, which, grown into non-adhering colonies called neurospheres, express nestin, a neurofilament marker. Analysis of hESC and hESC-derived neural stem cell nuclear extracts revealed an increased expression of Reptin52 in neurosphere nuclei. The increase in Reptin52 was evident throughout directed neuronal differentiation as assessed by western blotting, quantitative RT-PCR and immunocytochemistry. Reptin52 serves a pivotal regulatory role in nuclear activities such as transcription regulation and histone modification. In that regard, co-immunoprecipitation experiments showed that binding partners of Reptin52 (Pontin52, β-catenin and ATF-2) associate with this regulatory protein in hESC-derived neuronal precursors. Moreover, expression of two of these proteins (β-catenin - the end product of the Wnt signaling pathway - and ATF-2) is coordinately regulated with Reptin52.

UR - http://www.scopus.com/inward/record.url?scp=59349093511&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=59349093511&partnerID=8YFLogxK

U2 - 10.1016/j.neulet.2009.01.023

DO - 10.1016/j.neulet.2009.01.023

M3 - Article

C2 - 19444951

AN - SCOPUS:59349093511

VL - 452

SP - 47

EP - 51

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 1

ER -