@article{60908ab55afb44d4a91f837ee6bd729b,
title = "Repurposing a photosynthetic antenna protein as a super-resolution microscopy label",
abstract = "Techniques such as Stochastic Optical Reconstruction Microscopy (STORM) and Structured Illumination Microscopy (SIM) have increased the achievable resolution of optical imaging, but few fluorescent proteins are suitable for super-resolution microscopy, particularly in the far-red and near-infrared emission range. Here we demonstrate the applicability of CpcA, a subunit of the photosynthetic antenna complex in cyanobacteria, for STORM and SIM imaging. The periodicity and width of fabricated nanoarrays of CpcA, with a covalently attached phycoerythrobilin (PEB) or phycocyanobilin (PCB) chromophore, matched the lines in reconstructed STORM images. SIM and STORM reconstructions of Escherichia coli cells harbouring CpcA-labelled cytochrome bd 1 ubiquinol oxidase in the cytoplasmic membrane show that CpcA-PEB and CpcA-PCB are suitable for super-resolution imaging in vivo. The stability, ease of production, small size and brightness of CpcA-PEB and CpcA-PCB demonstrate the potential of this largely unexplored protein family as novel probes for super-resolution microscopy.",
author = "Barnett, {Samuel F.H.} and Andrew Hitchcock and Mandal, {Amit K.} and Cvetelin Vasilev and Yuen, {Jonathan M.} and James Morby and Brindley, {Amanda A.} and Niedzwiedzki, {Dariusz M.} and Bryant, {Donald A.} and Cadby, {Ashley J.} and Dewey Holten and Hunter, {C. Neil}",
note = "Funding Information: This work was supported as part of the Photosynthetic Antenna Research Center (PARC), an Energy Frontier Research Center funded by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences under Award Number DE-SC 0001035. PARC supported photophysical studies of CpcA proteins (A.K.M., J.M.Y., D.M.N. and D.H.), nanopatterning (C.V.) and provided partial support for C.N.H. and D.A.B. D. A. B. also acknowledges financial support from the U. S. National Science Foundation (MCB-1613022). C.N.H. also acknowledges financial support from Advanced Award 338895 from the European Research Council. C.N.H., A.H., A.A.B. and A.J.C. acknowledge financial support from the Biotechnology and Biological Sciences Research Council (BBSRC UK), award number BB/M000265/1. S.F.H.B and J.M.M. were supported by doctoral studentships from the BBSRC. The SIM imaging was performed at the University of Sheffield Wolfson Light Microscopy Facility and was partly funded by MRC grant MR/K015753/1.",
year = "2017",
month = dec,
day = "1",
doi = "10.1038/s41598-017-16834-z",
language = "English (US)",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",
}