Requirement of km23 for TGFβ-mediated growth inhibition and induction of fibronectin expression

Qunyan Jin, Wei Ding, Cory M. Staub, Guofeng Gao, Qian Tang, Kathleen M. Mulder

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We previously identified km23 as a novel TGFβ receptor-interacting protein. Here we show that km23 is ubiquitously expressed in human tissues and that cell-type specific differences in endogenous km23 protein expression exist. In addition, we demonstrate that the phosphorylation of km23 is TGFβ-dependent, in that EGF was unable to phosphorylate km23. Further, the kinase activity of both TGFβ receptors appears to play a role in the TGFβ-mediated phosphorylation of km23, although TGFβ RII kinase activity is absolutely required for km23 phosphorylation. Blockade of km23 using small interfering RNAs significantly decreased key TGFβ responses, including induction of fibronectin expression and inhibition of cell growth. Thus, our results demonstrate that km23 is required for TGFβ induction of fibronectin expression and is necessary, but not sufficient, for TGFβ-mediated growth inhibition.

Original languageEnglish (US)
Pages (from-to)1363-1372
Number of pages10
JournalCellular Signalling
Volume17
Issue number11
DOIs
StatePublished - Nov 1 2005

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Fibronectins
Phosphorylation
Phosphotransferases
Growth
Receptor-Interacting Protein Serine-Threonine Kinases
Epidermal Growth Factor
Small Interfering RNA
Proteins

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Jin, Qunyan ; Ding, Wei ; Staub, Cory M. ; Gao, Guofeng ; Tang, Qian ; Mulder, Kathleen M. / Requirement of km23 for TGFβ-mediated growth inhibition and induction of fibronectin expression. In: Cellular Signalling. 2005 ; Vol. 17, No. 11. pp. 1363-1372.
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Requirement of km23 for TGFβ-mediated growth inhibition and induction of fibronectin expression. / Jin, Qunyan; Ding, Wei; Staub, Cory M.; Gao, Guofeng; Tang, Qian; Mulder, Kathleen M.

In: Cellular Signalling, Vol. 17, No. 11, 01.11.2005, p. 1363-1372.

Research output: Contribution to journalArticle

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