Rescue of γ2 subunit-deficient mice by transgenic overexpression of the GABA(A) receptor γ2S or γ2L subunit isoforms

Kristin Baer, Christian Essrich, Sylvia Balsiger, Marilee J. Wick, R. Adron Harris, Jean Marc Fritschy, Bernhard Luscher

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The γ2 subunit is an important functional determinant of GABA(A) receptors and is essential for formation of high-affinity benzodiazepine binding sites and for synaptic clustering of major GABA(A) receptor subtypes along with gephyrin. There are two splice variants of the γ2 subunit, γ2 short (γ2S) and γ2 long (γ2L), the latter carrying in the cytoplasmic domain an additional eight amino acids with a putative phosphorylation site. Here, we show that transgenic mice expressing either the γ2S or γ2L subunit on a γ2 subunit-deficient background are phenotypically indistinguishable from wild-type. They express nearly normal levels of γ2 subunit protein and [3H]flumazenil binding sites. Likewise, the distribution, number and size of GABA(A) receptor clusters colocalized with gephyrin are similar to wild-type in both juvenile and adult mice. Our results indicate that the two γ2 subunit splice variants can substitute for each other and fulfil the basic functions of GABA(A) receptors, allowing in vivo studies that address isoform-specific roles in phosphorylation-dependent regulatory mechanisms.

Original languageEnglish (US)
Pages (from-to)2639-2643
Number of pages5
JournalEuropean Journal of Neuroscience
Volume12
Issue number7
DOIs
StatePublished - Jan 1 2000

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GABA-A Receptors
Transgenic Mice
Protein Isoforms
Binding Sites
Phosphorylation
Flumazenil
Protein Subunits
Benzodiazepines
Cluster Analysis
Amino Acids
gephyrin

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Baer, Kristin ; Essrich, Christian ; Balsiger, Sylvia ; Wick, Marilee J. ; Harris, R. Adron ; Fritschy, Jean Marc ; Luscher, Bernhard. / Rescue of γ2 subunit-deficient mice by transgenic overexpression of the GABA(A) receptor γ2S or γ2L subunit isoforms. In: European Journal of Neuroscience. 2000 ; Vol. 12, No. 7. pp. 2639-2643.
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Rescue of γ2 subunit-deficient mice by transgenic overexpression of the GABA(A) receptor γ2S or γ2L subunit isoforms. / Baer, Kristin; Essrich, Christian; Balsiger, Sylvia; Wick, Marilee J.; Harris, R. Adron; Fritschy, Jean Marc; Luscher, Bernhard.

In: European Journal of Neuroscience, Vol. 12, No. 7, 01.01.2000, p. 2639-2643.

Research output: Contribution to journalArticle

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