Rescue of infectious particles from preassembled alphavirus nucleocapsid cores

Jonathan E. Snyder, Odisse Azizgolshani, Bingbing Wu, Yingpei He, Aih Cheun Lee, Joyce Jose, Daniel M. Suter, Charles M. Knobler, William M. Gelbart, Richard J. Kuhn

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Alphaviruses are small, spherical, enveloped, positive-sense, single-stranded, RNA viruses responsible for considerable human and animal disease. Using microinjection of preassembled cores as a tool, a system has been established to study the assembly and budding process of Sindbis virus, the type member of the alphaviruses. We demonstrate the release of infectious virus-like particles from cells expressing Sindbis virus envelope glycoproteins following microinjection of Sindbis virus nucleocapsids purified from the cytoplasm of infected cells. Furthermore, it is shown that nucleocapsids assembled in vitro mimic those isolated in the cytoplasm of infected cells with respect to their ability to be incorporated into enveloped virions following microinjection. This system allows for the study of the alphavirus budding process independent of an authentic infection and provides a platform to study viral and host requirements for budding.

Original languageEnglish (US)
Pages (from-to)5773-5781
Number of pages9
JournalJournal of virology
Volume85
Issue number12
DOIs
StatePublished - Jun 1 2011

Fingerprint

Sindbis virus
Sindbis Virus
Alphavirus
Nucleocapsid
nucleocapsid
Microinjections
Virion
positive sense, single-stranded RNA viruses
Cytoplasm
cytoplasm
diseases and disorders (animals and humans)
Animal Diseases
virus-like particles
RNA Viruses
cells
virion
glycoproteins
Glycoproteins
Infection
infection

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Snyder, J. E., Azizgolshani, O., Wu, B., He, Y., Lee, A. C., Jose, J., ... Kuhn, R. J. (2011). Rescue of infectious particles from preassembled alphavirus nucleocapsid cores. Journal of virology, 85(12), 5773-5781. https://doi.org/10.1128/JVI.00039-11
Snyder, Jonathan E. ; Azizgolshani, Odisse ; Wu, Bingbing ; He, Yingpei ; Lee, Aih Cheun ; Jose, Joyce ; Suter, Daniel M. ; Knobler, Charles M. ; Gelbart, William M. ; Kuhn, Richard J. / Rescue of infectious particles from preassembled alphavirus nucleocapsid cores. In: Journal of virology. 2011 ; Vol. 85, No. 12. pp. 5773-5781.
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Snyder, JE, Azizgolshani, O, Wu, B, He, Y, Lee, AC, Jose, J, Suter, DM, Knobler, CM, Gelbart, WM & Kuhn, RJ 2011, 'Rescue of infectious particles from preassembled alphavirus nucleocapsid cores', Journal of virology, vol. 85, no. 12, pp. 5773-5781. https://doi.org/10.1128/JVI.00039-11

Rescue of infectious particles from preassembled alphavirus nucleocapsid cores. / Snyder, Jonathan E.; Azizgolshani, Odisse; Wu, Bingbing; He, Yingpei; Lee, Aih Cheun; Jose, Joyce; Suter, Daniel M.; Knobler, Charles M.; Gelbart, William M.; Kuhn, Richard J.

In: Journal of virology, Vol. 85, No. 12, 01.06.2011, p. 5773-5781.

Research output: Contribution to journalArticle

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