Research on regulation function of γ-secretase inhibitor DAPT on the differentiation of neural precursor cell line

Qi Wang, Hedong Li

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

This study aims to investigate the effect of γ-Secretase Inhibitor DAPT, (N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester), on the differentiation of neural precursor cells and the production of neurons in the neural precursor cell line GE6. GE6 was cultured in medium with 4 μmol/L DAPT added as the experimental group and the untreated medium separately as the control group. After 4 days of differentiation, we carried out the following experiments. We used immuno-fluorescent staining to observe the ratio of Tuj1, GFAP and O4 positive cells. We also used qRT-PCR to detect the effect of the DAPT on Tuj1 and GFAP mRNA transcription in the GE6. The results of immuno-fluorescent staining indicated that the Tuj1 ratio of experimental group was higher compared to that of the control group, but the GFAP and O4 ratio of experimental group was lower than that of the control group. The differences were statistically significant (P < 0.05). The result of qRT-PCR was in accordance with immunofluorescent staining results. It was well concluded that DAPT could promote the neurogenic differentiation of neural precursor cell line rather than leading to gliogenic differentiation. More neurons could be obtained for transplantation with the addition of DAPT.

Original languageEnglish (US)
Pages (from-to)126-130
Number of pages5
JournalSheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi
Volume32
Issue number1
StatePublished - Feb 1 2015

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Research on regulation function of γ-secretase inhibitor DAPT on the differentiation of neural precursor cell line'. Together they form a unique fingerprint.

Cite this