Resident murine alveolar and peritoneal macrophages differ in adhesion of apoptotic thymocytes

Bin Hu, Jeffrey H. Jennings, Joanne Sonstein, Joanna Floros, Jill C. Todt, Timothy Polak, Jeffrey L. Curtis

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Apoptotic cells must be cleared efficiently by macrophages (Mø) to prevent autoimmunity, yet their ingestion impairs Mø microbicidal function. The principal murine resident lung phagocyte, the alveolar Mø (AMø), is specifically deficient at apoptotic cell ingestion, both in vitro and in vivo, compared with resident peritoneal Mø (PMø). To further characterize this deficiency, we assayed static adhesion in vitro using apoptotic thymocytes and resident AMø and PMø from normal C57BL/6 mice. Adhesion of apoptotic thymocytes by both types of Mø was rapid, specific, and cold-sensitive. Antibody against the receptor tyrosine kinase MerTK (Tyro12) blocked phagocytosis but not adhesion in both types of Mø. Surfactant protein A increased adhesion and phagocytosis by AMø, but not to the levels seen using PMø. Adhesion was largely cation-independent for PMø and calcium-dependent for AMø. Adhesion was not inhibited in either Mø type by mAbs against β1 or β3 integrins or scavenger receptor I/II (CD204), but AMø adhesion was inhibited by specific mAbs against CD11c/CD18. Thus, resident murine tissue Mø from different tissues depend on qualitatively disparate receptor systems to bind apoptotic cells. The decreased capacity of murine AMø to ingest apoptotic cells is only partially explained by reduced initial adhesion.

Original languageEnglish (US)
Pages (from-to)687-693
Number of pages7
JournalAmerican journal of respiratory cell and molecular biology
Volume30
Issue number5
DOIs
StatePublished - May 1 2004

Fingerprint

Macrophages
Alveolar Macrophages
Peritoneal Macrophages
Thymocytes
Adhesion
Phagocytosis
Eating
Cells
Pulmonary Surfactant-Associated Protein A
Scavenger Receptors
Receptor Protein-Tyrosine Kinases
Phagocytes
Autoimmunity
Inbred C57BL Mouse
Integrins
Cations
Tissue
Calcium
Lung
Antibodies

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

Cite this

Hu, Bin ; Jennings, Jeffrey H. ; Sonstein, Joanne ; Floros, Joanna ; Todt, Jill C. ; Polak, Timothy ; Curtis, Jeffrey L. / Resident murine alveolar and peritoneal macrophages differ in adhesion of apoptotic thymocytes. In: American journal of respiratory cell and molecular biology. 2004 ; Vol. 30, No. 5. pp. 687-693.
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Resident murine alveolar and peritoneal macrophages differ in adhesion of apoptotic thymocytes. / Hu, Bin; Jennings, Jeffrey H.; Sonstein, Joanne; Floros, Joanna; Todt, Jill C.; Polak, Timothy; Curtis, Jeffrey L.

In: American journal of respiratory cell and molecular biology, Vol. 30, No. 5, 01.05.2004, p. 687-693.

Research output: Contribution to journalArticle

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