Resistant and susceptible cacao genotypes exhibit defense gene polymorphism and unique early responses to Phytophthora megakarya inoculation

Désiré N. Pokou, Andrew S. Fister, Noah Winters, Mathias Tahi, Coulibaly Klotioloma, Aswathy Sebastian, James Harold Marden, Siela Maximova, Mark Guiltinan

Research output: Contribution to journalArticle

Abstract

Key message: Key genes potentially involved in cacao disease resistance were identified by transcriptomic analysis ofimportant cacao cultivars. Defense gene polymorphisms were identified which could contribute topathogen recognition capacity. Abstract: Cacao suffers significant annual losses to the water mold Phytophthora spp. (Oomycetes). In West Africa, P. megakarya poses a major threat to farmer livelihood and the stability of cocoa production. As part of a long-term goal to define key disease resistance genes in cacao, here we use a transcriptomic analysis of the disease-resistant cacao clone SCA6 and the susceptible clone NA32 to characterize basal differences in gene expression, early responses to infection, and polymorphisms in defense genes. Gene expression measurements by RNA-seq along a time course revealed the strongest transcriptomic response 24 h after inoculation in the resistant genotype. We observed strong regulation of several pathogenesis-related genes, pattern recognition receptors, and resistance genes, which could be critical for the ability of SCA6 to combat infection. These classes of genes also showed differences in basal expression between the two genotypes prior to infection, suggesting that prophylactic expression of defense-associated genes could contribute to SCA6’s broad-spectrum disease resistance. Finally, we analyzed polymorphism in a set of defense-associated receptors, identifying coding variants between SCA6 and NA32 which could contribute to unique capacities for pathogen recognition. This work is an important step toward characterizing genetic differences underlying a successful defense response in cacao.

Original languageEnglish (US)
Pages (from-to)499-516
Number of pages18
JournalPlant molecular biology
Volume99
Issue number4-5
DOIs
StatePublished - Mar 1 2019

Fingerprint

Phytophthora
Cacao
Genotype
genetic polymorphism
genotype
Genes
Disease Resistance
genes
transcriptomics
disease resistance
Clone Cells
Infection
Oomycetes
infection
clones
Pattern Recognition Receptors
Gene Expression
gene expression
receptors
Western Africa

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Pokou, Désiré N. ; Fister, Andrew S. ; Winters, Noah ; Tahi, Mathias ; Klotioloma, Coulibaly ; Sebastian, Aswathy ; Marden, James Harold ; Maximova, Siela ; Guiltinan, Mark. / Resistant and susceptible cacao genotypes exhibit defense gene polymorphism and unique early responses to Phytophthora megakarya inoculation. In: Plant molecular biology. 2019 ; Vol. 99, No. 4-5. pp. 499-516.
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Resistant and susceptible cacao genotypes exhibit defense gene polymorphism and unique early responses to Phytophthora megakarya inoculation. / Pokou, Désiré N.; Fister, Andrew S.; Winters, Noah; Tahi, Mathias; Klotioloma, Coulibaly; Sebastian, Aswathy; Marden, James Harold; Maximova, Siela; Guiltinan, Mark.

In: Plant molecular biology, Vol. 99, No. 4-5, 01.03.2019, p. 499-516.

Research output: Contribution to journalArticle

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T1 - Resistant and susceptible cacao genotypes exhibit defense gene polymorphism and unique early responses to Phytophthora megakarya inoculation

AU - Pokou, Désiré N.

AU - Fister, Andrew S.

AU - Winters, Noah

AU - Tahi, Mathias

AU - Klotioloma, Coulibaly

AU - Sebastian, Aswathy

AU - Marden, James Harold

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AU - Guiltinan, Mark

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