Resolution of myocardial myosin light chains by two dimensional gel electrophoresis

J. McPherson, R. R. Traut, D. T. Mason, R. Zelis, J. Wikman-Coffelt

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Two dimensional gel electrophoresis of canine myocardial light chains of myosin demonstrated the existence of a single polypeptide species corresponding to the lighter component, C 2. However, contrary to previous observations, it was found that the heavier polypeptide, C 1, although homogeneous by one dimensional electrophoresis in polyacrylamide gels containing dodecyl sulfate, is resolved into 4 components by two dimensional gel electrophoresis at pH 8.7 and 4.5. Of these 4 components 2, named C1c and C1d, present in roughly equal amounts, comprise approximately 95% of the total C1 fraction, while the remaining 5% of the C 1 material consists of 2 components, named C1a and C1b.

Original languageEnglish (US)
Pages (from-to)994-996
Number of pages3
JournalJournal of Biological Chemistry
Volume249
Issue number3
StatePublished - Jan 1 1974

Fingerprint

Myosin Light Chains
Electrophoresis, Gel, Two-Dimensional
Electrophoresis
Gels
Canidae
Polyacrylamide Gel Electrophoresis
Peptides
dodecyl sulfate
polypeptide C

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

McPherson, J., Traut, R. R., Mason, D. T., Zelis, R., & Wikman-Coffelt, J. (1974). Resolution of myocardial myosin light chains by two dimensional gel electrophoresis. Journal of Biological Chemistry, 249(3), 994-996.
McPherson, J. ; Traut, R. R. ; Mason, D. T. ; Zelis, R. ; Wikman-Coffelt, J. / Resolution of myocardial myosin light chains by two dimensional gel electrophoresis. In: Journal of Biological Chemistry. 1974 ; Vol. 249, No. 3. pp. 994-996.
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McPherson, J, Traut, RR, Mason, DT, Zelis, R & Wikman-Coffelt, J 1974, 'Resolution of myocardial myosin light chains by two dimensional gel electrophoresis', Journal of Biological Chemistry, vol. 249, no. 3, pp. 994-996.

Resolution of myocardial myosin light chains by two dimensional gel electrophoresis. / McPherson, J.; Traut, R. R.; Mason, D. T.; Zelis, R.; Wikman-Coffelt, J.

In: Journal of Biological Chemistry, Vol. 249, No. 3, 01.01.1974, p. 994-996.

Research output: Contribution to journalArticle

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