Response of lab-scale methanogenic reactors inoculated from different sources to organic loading rate shocks

Lisa M. Steinberg, John Ragan

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Anaerobic digester failure due to a pH drop may be overcome with the use of an acidotolerant methanogenic community. To test this, lab-scale reactors were inoculated from acidic bog sediments, a municipal sludge digester, or a combination of these inocula and challenged with glucose pulses without pH control. Only the bog reactor survived the first glucose shock, and the methanogen community was dominated by members of the acidic Fen Cluster. After restarting the digester and hybrid reactors, two subsequent glucose shocks were applied. Methanogenic communities converged in all reactors and were dominated by Methanosarcina and Methanobacteriaceae. The Fen Cluster was eventually nondetectable in bog and hybrid reactors, presumably due to periods of circumneutral pH with only intermittent periods of low pH following glucose shocks. Although the resultant communities required base addition, an increase in Methanosarcina numbers after glucose pulses resulted in decreased acetate and increased reactor pH and methane production.

Original languageEnglish (US)
Pages (from-to)8790-8798
Number of pages9
JournalBioresource technology
Volume102
Issue number19
DOIs
StatePublished - Oct 1 2011

Fingerprint

Glucose
glucose
bog
fen
Methanogens
Methane
Sediments
Acetates
reactor
rate
acetate
methane
sludge
sediment
digester

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

@article{88cf547d02fe4934b41891d736d88930,
title = "Response of lab-scale methanogenic reactors inoculated from different sources to organic loading rate shocks",
abstract = "Anaerobic digester failure due to a pH drop may be overcome with the use of an acidotolerant methanogenic community. To test this, lab-scale reactors were inoculated from acidic bog sediments, a municipal sludge digester, or a combination of these inocula and challenged with glucose pulses without pH control. Only the bog reactor survived the first glucose shock, and the methanogen community was dominated by members of the acidic Fen Cluster. After restarting the digester and hybrid reactors, two subsequent glucose shocks were applied. Methanogenic communities converged in all reactors and were dominated by Methanosarcina and Methanobacteriaceae. The Fen Cluster was eventually nondetectable in bog and hybrid reactors, presumably due to periods of circumneutral pH with only intermittent periods of low pH following glucose shocks. Although the resultant communities required base addition, an increase in Methanosarcina numbers after glucose pulses resulted in decreased acetate and increased reactor pH and methane production.",
author = "Steinberg, {Lisa M.} and John Ragan",
year = "2011",
month = "10",
day = "1",
doi = "10.1016/j.biortech.2011.07.017",
language = "English (US)",
volume = "102",
pages = "8790--8798",
journal = "Bioresource Technology",
issn = "0960-8524",
publisher = "Elsevier Limited",
number = "19",

}

Response of lab-scale methanogenic reactors inoculated from different sources to organic loading rate shocks. / Steinberg, Lisa M.; Ragan, John.

In: Bioresource technology, Vol. 102, No. 19, 01.10.2011, p. 8790-8798.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Response of lab-scale methanogenic reactors inoculated from different sources to organic loading rate shocks

AU - Steinberg, Lisa M.

AU - Ragan, John

PY - 2011/10/1

Y1 - 2011/10/1

N2 - Anaerobic digester failure due to a pH drop may be overcome with the use of an acidotolerant methanogenic community. To test this, lab-scale reactors were inoculated from acidic bog sediments, a municipal sludge digester, or a combination of these inocula and challenged with glucose pulses without pH control. Only the bog reactor survived the first glucose shock, and the methanogen community was dominated by members of the acidic Fen Cluster. After restarting the digester and hybrid reactors, two subsequent glucose shocks were applied. Methanogenic communities converged in all reactors and were dominated by Methanosarcina and Methanobacteriaceae. The Fen Cluster was eventually nondetectable in bog and hybrid reactors, presumably due to periods of circumneutral pH with only intermittent periods of low pH following glucose shocks. Although the resultant communities required base addition, an increase in Methanosarcina numbers after glucose pulses resulted in decreased acetate and increased reactor pH and methane production.

AB - Anaerobic digester failure due to a pH drop may be overcome with the use of an acidotolerant methanogenic community. To test this, lab-scale reactors were inoculated from acidic bog sediments, a municipal sludge digester, or a combination of these inocula and challenged with glucose pulses without pH control. Only the bog reactor survived the first glucose shock, and the methanogen community was dominated by members of the acidic Fen Cluster. After restarting the digester and hybrid reactors, two subsequent glucose shocks were applied. Methanogenic communities converged in all reactors and were dominated by Methanosarcina and Methanobacteriaceae. The Fen Cluster was eventually nondetectable in bog and hybrid reactors, presumably due to periods of circumneutral pH with only intermittent periods of low pH following glucose shocks. Although the resultant communities required base addition, an increase in Methanosarcina numbers after glucose pulses resulted in decreased acetate and increased reactor pH and methane production.

UR - http://www.scopus.com/inward/record.url?scp=80052372191&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052372191&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2011.07.017

DO - 10.1016/j.biortech.2011.07.017

M3 - Article

VL - 102

SP - 8790

EP - 8798

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

IS - 19

ER -