TY - JOUR
T1 - Response of Salivary Microbiota to Caries Preventive Treatment in Aboriginal and Torres Strait Islander Children
AU - Skelly, Emily
AU - Johnson, Newell W.
AU - Kapellas, Kostas
AU - Kroon, Jeroen
AU - Lalloo, Ratilal
AU - Weyrich, Laura
N1 - Funding Information:
The study was funded by the Australian National Health and Medical Research Council [Project Grant APP 1081320] for all fieldwork, and by DECRA grant from the Australian Research Council [DE150101574] and the Australian Dental Research Foundation grant [136-2016] for laboratory consumables and sequencing. The authors acknowledge and pay their respects to the traditional owners of the lands on which this research took place: the Anggamuthi, Atambay, Wuthathi, Yadhaykenu and Gudang peoples of the Northern Peninsular Area (NPA); the Kaurna people of the Adelaide Plains; the Yugambeh/Kombumerri peoples of the Gold Coast; and the Turrbal and Jagara people of the Brisbane (Meanjin) area. This study would not have been possible without the generous support and cooperation of the Elders, the Mayor and Councillors of the Northern Peninsula Area Authority, and members of the Area Community Health Services. Our sincerest thanks to the Principal and Heads of Campuses of the NPA State College for enabling our research, and most of all, we are grateful to the children and their families. We thank other members of the project team for their involvement and assistance in this research, especially Dr Sanjeewa Kularatna, Dr Ohnmar Tut, Prof. Lisa Jamieson, Prof. Paul Scuffham, and our Indigenous colleagues, Valda Wallace and Yvonne Cadet-James for advice. We thank A/Prof. Robyn Boase, A/Prof. Don Gilchrist, and Helen Mills for being part of the team performing epidemiological screenings and sample collection. We thank Wendy Bell for her excellent project management, as well as the team responsible for rendering children in need dentally fit: Dr Joel Rogers, Elizabeth Cobbledick, Carole Williams, and Amber Sullivan. We thank Dr David Speicher for advice on sample collection and protocols, and Dr Paul Gooding for all his help with sequencing through AGRF.
Publisher Copyright:
© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
PY - 2020/1/1
Y1 - 2020/1/1
N2 - A once-annual caries preventive (Intervention) treatment was offered to Aboriginal and Torres Strait Islander schoolchildren—a population with disproportionately poorer oral health than non-Indigenous Australian children—in the Northern Peninsula Area (NPA) of Far North Queensland (FNQ), which significantly improved their oral health. Here, we examine the salivary microbiota of these children (mean age = 10 ± 2.96 years; n = 103), reconstructing the bacterial community composition with high-throughput sequencing of the V4 region of bacterial 16S rRNA gene. Microbial communities of children who received the Intervention had lower taxonomic diversity than those who did not receive treatment (Shannon, p < 0.05). Moreover, the Intervention resulted in further decreased microbial diversity in children with active carious lesions existing at the time of saliva collection. Microbial species associated with caries were detected; Lactobacillus salivarius, Lactobacillus reuteri, Lactobacillus gasseri, Prevotella multisaccharivorax, Parascardovia denticolens, and Mitsuokella HMT 131 were significantly increased (p < 0.05) in children with severe caries, especially in children who did not receive the Intervention. These insights into microbial associations and community differences prompt future considerations to the mechanisms behind caries-preventive therapy induced change; important for understanding the long-term implications of like treatment to improve oral health disparities within Australia. Trial registration: ANZCTR, ACTRN12615000693527. Registered 3 July 2015, https://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=368750&isReview=true.
AB - A once-annual caries preventive (Intervention) treatment was offered to Aboriginal and Torres Strait Islander schoolchildren—a population with disproportionately poorer oral health than non-Indigenous Australian children—in the Northern Peninsula Area (NPA) of Far North Queensland (FNQ), which significantly improved their oral health. Here, we examine the salivary microbiota of these children (mean age = 10 ± 2.96 years; n = 103), reconstructing the bacterial community composition with high-throughput sequencing of the V4 region of bacterial 16S rRNA gene. Microbial communities of children who received the Intervention had lower taxonomic diversity than those who did not receive treatment (Shannon, p < 0.05). Moreover, the Intervention resulted in further decreased microbial diversity in children with active carious lesions existing at the time of saliva collection. Microbial species associated with caries were detected; Lactobacillus salivarius, Lactobacillus reuteri, Lactobacillus gasseri, Prevotella multisaccharivorax, Parascardovia denticolens, and Mitsuokella HMT 131 were significantly increased (p < 0.05) in children with severe caries, especially in children who did not receive the Intervention. These insights into microbial associations and community differences prompt future considerations to the mechanisms behind caries-preventive therapy induced change; important for understanding the long-term implications of like treatment to improve oral health disparities within Australia. Trial registration: ANZCTR, ACTRN12615000693527. Registered 3 July 2015, https://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=368750&isReview=true.
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U2 - 10.1080/20002297.2020.1830623
DO - 10.1080/20002297.2020.1830623
M3 - Article
C2 - 33149844
AN - SCOPUS:85092468568
SN - 2000-2297
VL - 12
JO - Journal of Oral Microbiology
JF - Journal of Oral Microbiology
IS - 1
M1 - 1830623
ER -