Response surface optimization of the cultivation conditions and medium composition a novel probiotic strain Bacillus pumilus STF26

D. Han, N. O. San, P. Angun, O. C. Onarman Umu, A. Demirci, T. Tekinay

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Probiotics have been widely used in aquaculture as a controlling disease, enhancing immune responses, providing nutrients, and improving water quality. The use of Bacillus species as probiotics is expanding rapidly with increasing number of studies. Optimization of cultivation conditions and the medium composition for the improvement of novel potential probiotic strain Bacillus pumilus STF26 was performed by using response surface methodology (RSM). Factors optimized were temperature, pH and the concentrations of dextrose, yeast extract, KH2PO4 and MgSO4.7H2O. The optimum values are found as 30.9°C, 6.9, 20% (w/v), for temperature, pH and the concentrations of dextrose and 1.526% (w/v), 0.1% (w/v) and 0.5% (w/v), for yeast extract, KH2PO4 and MgSO4.7H2O, respectively. As a result, maximum biomass at optimum conditions was 10.42 g/L, which is nearly 2.5 times higher when compared to the one obtained by using Luria Broth medium at optimized temperature and pH values.

Original languageEnglish (US)
Pages (from-to)1355-1361
Number of pages7
JournalInternational Food Research Journal
Volume21
Issue number4
StatePublished - 2014

All Science Journal Classification (ASJC) codes

  • Food Science

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