Responses of proenkephalin Peptide F to aerobic exercise stress in the plasma and white blood cell biocompartments

William J. Kraemer, Maren S. Fragala, Wendy R.H.Beijersbergen Van Henegouwen, Scott E. Gordon, Jill A. Bush, Jeff S. Volek, N. Travis Triplett, Courtenay Dunn-Lewis, Brett A. Comstock, Tunde K. Szivak, Shawn D. Flanagan, David R. Hooper, Hui Ying Luk, Andrea Marie Mastro

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Proenkephalin Peptide F [107-140] is an enkephalin-containing peptide found predominantly within the adrenal medulla, co-packaged with epinephrine within the chromaffin granules. In vivo studies indicate that Peptide F has classic opioid analgesia effects; in vitro studies suggest potential immune cell interactions. In this investigation we examined patterns of Peptide F concentrations in different bio-compartments of the blood at rest and following sub-maximal cycle exercise to determine if Peptide F interacts with the white blood cell (WBC) bio-compartment during aerobic exercise. Eight physically active men (n = 8) performed sub-maximal (80-85% V̇O2Speak) cycle ergometer exercise for 30 min. Plasma Peptide F and WBC Peptide F immunoreactivity were examined pre-exercise, mid-exercise and immediately post-, 5-min post-, 15-min post-, 30-min post- and 60-min post-exercise and at similar time-points during a control condition (30 min rest). Peptide F concentrations significantly (p < 0.05) increased at 5 and 60 min post-exercise, compared to pre-exercise concentrations. No significant increases in Peptide F concentrations in the WBC fraction were observed during or after exercise. However, a significant decrease was observed at 30 min post-exercise. An ultradian pattern of Peptide F distribution was apparent during rest. Furthermore, concentrations of T cells, B cells, NK cells, and total WBCs demonstrated significant changes in response to aerobic exercise. Data indicated that Peptide F was bound in significant molar concentrations in the WBC fraction and that this biocompartment may be one of the tissue targets for binding interactions. These data indicate that Peptide F is involved with immune cell modulation in the white blood circulatory biocompartment of blood.

Original languageEnglish (US)
Pages (from-to)118-124
Number of pages7
JournalPeptides
Volume42
DOIs
StatePublished - Apr 1 2013

Fingerprint

Leukocytes
Blood
Cells
Exercise
Plasmas
peptide F
Chromaffin Granules
Exercise equipment
Adrenal Medulla
T-cells
Enkephalins
Cell Communication
Natural Killer Cells
Analgesia
Opioid Analgesics
Epinephrine
B-Lymphocytes
Modulation
Tissue
T-Lymphocytes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

Cite this

Kraemer, W. J., Fragala, M. S., Van Henegouwen, W. R. H. B., Gordon, S. E., Bush, J. A., Volek, J. S., ... Mastro, A. M. (2013). Responses of proenkephalin Peptide F to aerobic exercise stress in the plasma and white blood cell biocompartments. Peptides, 42, 118-124. https://doi.org/10.1016/j.peptides.2013.01.013
Kraemer, William J. ; Fragala, Maren S. ; Van Henegouwen, Wendy R.H.Beijersbergen ; Gordon, Scott E. ; Bush, Jill A. ; Volek, Jeff S. ; Triplett, N. Travis ; Dunn-Lewis, Courtenay ; Comstock, Brett A. ; Szivak, Tunde K. ; Flanagan, Shawn D. ; Hooper, David R. ; Luk, Hui Ying ; Mastro, Andrea Marie. / Responses of proenkephalin Peptide F to aerobic exercise stress in the plasma and white blood cell biocompartments. In: Peptides. 2013 ; Vol. 42. pp. 118-124.
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abstract = "Proenkephalin Peptide F [107-140] is an enkephalin-containing peptide found predominantly within the adrenal medulla, co-packaged with epinephrine within the chromaffin granules. In vivo studies indicate that Peptide F has classic opioid analgesia effects; in vitro studies suggest potential immune cell interactions. In this investigation we examined patterns of Peptide F concentrations in different bio-compartments of the blood at rest and following sub-maximal cycle exercise to determine if Peptide F interacts with the white blood cell (WBC) bio-compartment during aerobic exercise. Eight physically active men (n = 8) performed sub-maximal (80-85{\%} V̇O2Speak) cycle ergometer exercise for 30 min. Plasma Peptide F and WBC Peptide F immunoreactivity were examined pre-exercise, mid-exercise and immediately post-, 5-min post-, 15-min post-, 30-min post- and 60-min post-exercise and at similar time-points during a control condition (30 min rest). Peptide F concentrations significantly (p < 0.05) increased at 5 and 60 min post-exercise, compared to pre-exercise concentrations. No significant increases in Peptide F concentrations in the WBC fraction were observed during or after exercise. However, a significant decrease was observed at 30 min post-exercise. An ultradian pattern of Peptide F distribution was apparent during rest. Furthermore, concentrations of T cells, B cells, NK cells, and total WBCs demonstrated significant changes in response to aerobic exercise. Data indicated that Peptide F was bound in significant molar concentrations in the WBC fraction and that this biocompartment may be one of the tissue targets for binding interactions. These data indicate that Peptide F is involved with immune cell modulation in the white blood circulatory biocompartment of blood.",
author = "Kraemer, {William J.} and Fragala, {Maren S.} and {Van Henegouwen}, {Wendy R.H.Beijersbergen} and Gordon, {Scott E.} and Bush, {Jill A.} and Volek, {Jeff S.} and Triplett, {N. Travis} and Courtenay Dunn-Lewis and Comstock, {Brett A.} and Szivak, {Tunde K.} and Flanagan, {Shawn D.} and Hooper, {David R.} and Luk, {Hui Ying} and Mastro, {Andrea Marie}",
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Kraemer, WJ, Fragala, MS, Van Henegouwen, WRHB, Gordon, SE, Bush, JA, Volek, JS, Triplett, NT, Dunn-Lewis, C, Comstock, BA, Szivak, TK, Flanagan, SD, Hooper, DR, Luk, HY & Mastro, AM 2013, 'Responses of proenkephalin Peptide F to aerobic exercise stress in the plasma and white blood cell biocompartments', Peptides, vol. 42, pp. 118-124. https://doi.org/10.1016/j.peptides.2013.01.013

Responses of proenkephalin Peptide F to aerobic exercise stress in the plasma and white blood cell biocompartments. / Kraemer, William J.; Fragala, Maren S.; Van Henegouwen, Wendy R.H.Beijersbergen; Gordon, Scott E.; Bush, Jill A.; Volek, Jeff S.; Triplett, N. Travis; Dunn-Lewis, Courtenay; Comstock, Brett A.; Szivak, Tunde K.; Flanagan, Shawn D.; Hooper, David R.; Luk, Hui Ying; Mastro, Andrea Marie.

In: Peptides, Vol. 42, 01.04.2013, p. 118-124.

Research output: Contribution to journalArticle

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AU - Kraemer, William J.

AU - Fragala, Maren S.

AU - Van Henegouwen, Wendy R.H.Beijersbergen

AU - Gordon, Scott E.

AU - Bush, Jill A.

AU - Volek, Jeff S.

AU - Triplett, N. Travis

AU - Dunn-Lewis, Courtenay

AU - Comstock, Brett A.

AU - Szivak, Tunde K.

AU - Flanagan, Shawn D.

AU - Hooper, David R.

AU - Luk, Hui Ying

AU - Mastro, Andrea Marie

PY - 2013/4/1

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N2 - Proenkephalin Peptide F [107-140] is an enkephalin-containing peptide found predominantly within the adrenal medulla, co-packaged with epinephrine within the chromaffin granules. In vivo studies indicate that Peptide F has classic opioid analgesia effects; in vitro studies suggest potential immune cell interactions. In this investigation we examined patterns of Peptide F concentrations in different bio-compartments of the blood at rest and following sub-maximal cycle exercise to determine if Peptide F interacts with the white blood cell (WBC) bio-compartment during aerobic exercise. Eight physically active men (n = 8) performed sub-maximal (80-85% V̇O2Speak) cycle ergometer exercise for 30 min. Plasma Peptide F and WBC Peptide F immunoreactivity were examined pre-exercise, mid-exercise and immediately post-, 5-min post-, 15-min post-, 30-min post- and 60-min post-exercise and at similar time-points during a control condition (30 min rest). Peptide F concentrations significantly (p < 0.05) increased at 5 and 60 min post-exercise, compared to pre-exercise concentrations. No significant increases in Peptide F concentrations in the WBC fraction were observed during or after exercise. However, a significant decrease was observed at 30 min post-exercise. An ultradian pattern of Peptide F distribution was apparent during rest. Furthermore, concentrations of T cells, B cells, NK cells, and total WBCs demonstrated significant changes in response to aerobic exercise. Data indicated that Peptide F was bound in significant molar concentrations in the WBC fraction and that this biocompartment may be one of the tissue targets for binding interactions. These data indicate that Peptide F is involved with immune cell modulation in the white blood circulatory biocompartment of blood.

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